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731. THERMOSYNTROPHA MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.30 g 0.294 1.689
2 0.30 g 0.294 3.945
3 0.50 g 0.49 8.388
4 1.00 g 0.98 18.328
5 0.30 g 0.294 1.447
6 0.05 g 0.049 0.333
7 Trace element solution 10.00 ml - -
8 10.00 g 9.804 -
9
(0.1% w/v)
0.50 ml 4.9e-4 0.002
10 3.00 g 2.941 35.011
11 3.00 g 2.941 27.75
12 Vitamin solution 10.00 ml - -
13 0.15 g 0.147 0.837
14 0.50 g 0.49 2.041
15 1000.00 ml 978.634 54322.4
Instructions:
  1. Dissolve ingredients except bicarbonate, carbonate, vitamins, cysteine and sulfide. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic, then dissolve carbonate and bicarbonate and adjust pH of medium to 8.5 using HCl. Dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Prior to inoculation add vitamins, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins should be sterilized by filtration.
Solution occurs in 192 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.50 g 1.5 7.848
2 3.00 g 3 12.172
3 0.50 g 0.5 2.958
4 1.00 g 1 17.111
5 0.10 g 0.1 0.36
6 0.18 g 0.18 0.64
7 0.10 g 0.1 0.68
8 0.18 g 0.18 0.626
9 0.01 g 0.01 0.04
10 0.02 g 0.02 0.042
11 0.01 g 0.01 0.162
12 0.01 g 0.01 0.041
13 0.03 g 0.03 0.126
14 0.30 mg 3.0e-4 0.001
15 0.40 mg 4.0e-4 0.001
16 1000.00 ml 998.207 55408.8
Instructions:
  1. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 8.5
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 100 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Yeast extract 9.804 -
NaHCO3 2.941 35.011
Na2CO3 2.941 27.75
NH4Cl 0.98 18.328
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain

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