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732a. DESULFUROMONAS MEDIUM (TCE)

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution A 902 ml - -
2 Solution B 100 ml - -
3 Solution C 10 ml - -
4 Solution D 1 ml - -
5 Solution E 10 ml - -
6 Solution F 10 ml - -
7 Solution G 15 ml - -
Instructions:
  1. Sparge solution A with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic serum vials (e.g., 9 ml in 50 ml bottles) and autoclave. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solutions C, D and F are autoclave under 100% N2 gas atmosphere.
  2. Add solution G only after inoculation of the medium!
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.65 g 0.721 4.137
2 0.17 g 0.188 1.366
3
(BD Bacto)
0.10 g 0.111 -
4 0.46 g 0.51 6.217
5 Selenite-tungstate solution 1.00 ml - -
6
(0.1% w/v)
0.50 ml 5.5e-4 0.002
7 900.00 ml 995.994 55286
8 , might need strain adjustment 2.50 g/l - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.27 g 2.7 28.099
2 3.73 g 37.3 444.013
3 100.00 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.11 g 11 74.822
2 0.10 g 10 49.188
3 10.00 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Trace element solution 1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Vitamin solution 9 ml - -
2 Seven vitamins solution 1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.3 g 30 124.905
2 10.0 ml 998.207 55408.8
Equipment needed: Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 13.5 ml 696.06 3073.9
2 1.5 ml 162.3 978.701
Instructions:
  1. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Prepare solution G by filling 13.5 ml hexadecane into a 50 ml serum bottle, then sparge with 100% N2 gas to make it anoxic and autoclave. Add 1.50 ml anoxic autoclaved tetrachloroethene to the sterile anoxic hexadecane solution by syringe. To complete the medium add appropriate amounts of solutions B to F to the sterile solution A in the sequence as indicated. The pH of the medium before inoculation should be at 7.2.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 4 other media (first defined in medium 732 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 1.487
2 2.0 g 2 10.06
3 70.0 mg 0.07 0.514
4 100.0 mg 0.1 0.505
5 6.0 mg 0.006 0.097
6 190.0 mg 0.19 0.799
7 2.0 mg 0.002 0.012
8 10.0 mg 0.01 0.075
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 1000.0 ml 998.207 55408.8
Instructions:
  1. First dissolve EDTA in distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add remaining compounds.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 86 other media (first defined in medium 503 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 100 mg 0.1 0.063
2 80 mg 0.08 0.583
3 20 mg 0.02 0.082
4 200 mg 0.2 1.625
5 100 mg 0.1 0.21
6 300 mg 0.3 1.459
7 200 mg 0.2 0.536
8 1000 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown
Equipment needed: Autoclave, Filter

Associated strains

All strains for this medium: 2
Strains with modifications: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 982.967 54562.9
Hexadecane 9.963 43.997
NaHCO3 3.559 42.368
Tetrachloroethene 2.323 14.008
K2HPO4 0.62 3.561
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
oxygen anaerobe 2 strains
temperature mesophilic 2 strains

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