Name: SULFITOBACTER PONTIACUS MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 733 #

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Na-acetate	20.00	mM	1.641	20
HEPES	8.00	g	7.992	33.538
K₂HPO₄	1.00	g	0.999	5.736
NH₄Cl	0.50	g	0.5	9.338
Yeast extract	1.00	g	0.999	-
Peptone	0.50	g	0.5	-
NaCl	15.00	g	14.985	256.417
MgSO₄ x 7 H₂O	1.00	g	0.999	4.053
CaCl₂ x 2 H₂O	0.05	g	0.05	0.34
Modified Wolin's mineral solution	1.00	ml	-	-
Biotin	0.10	g	0.1	0.409
Distilled water	1000.00	ml	-	-
1 Prepare the HEPES, NaCl, K₂HPO₄ and NH₄Cl as a basal salt solution, adjusting the pH to 7.5-7.8 with NaOH before sterilisation. All other components are added from sterile stock solutions.

Modified Wolin's mineral solution #

1000

Solution occurs in 166 other media (first defined in Medium 141).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Nitrilotriacetic acid	1.50	g	1.5	7.848
MgSO₄ x 7 H₂O	3.00	g	3	12.172
MnSO₄ x H₂O	0.50	g	0.5	2.958
NaCl	1.00	g	1	17.112
FeSO₄ x 7 H₂O	0.10	g	0.1	0.36
CoSO₄ x 7 H₂O	0.18	g	0.18	1.161
CaCl₂ x 2 H₂O	0.10	g	0.1	0.68
ZnSO₄ x 7 H₂O	0.18	g	0.18	0.626
CuSO₄ x 5 H₂O	0.01	g	0.01	0.04
AlK(SO₄)₂ x 12 H₂O	0.02	g	0.02	0.042
H₃BO₃	0.01	g	0.01	0.162
Na₂MoO₄ x 2 H₂O	0.01	g	0.01	0.041
NiCl₂ x 6 H₂O	0.03	g	0.03	0.231
Na₂SeO₃ x 5 H₂O	0.30	mg	3.0e-4	0.001
Na₂WO₄ x 2 H₂O	0.40	mg	4.0e-4	0.001
Distilled water	1000.00	ml	-	-
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

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