Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Na-acetate | 20.00 | mM | ||
|
HEPES | 8.00 | g | ||
|
K2HPO4 | 1.00 | g | ||
|
NH4Cl | 0.50 | g | ||
|
Yeast extract | 1.00 | g | ||
|
Peptone | 0.50 | g | ||
|
NaCl | 15.00 | g | ||
|
MgSO4 x 7 H2O | 1.00 | g | ||
|
CaCl2 x 2 H2O | 0.05 | g | ||
|
Modified Wolin's mineral solution | 1.00 | ml | ||
|
Biotin | 0.10 | g | ||
|
Distilled water | 1000.00 | ml | ||
1 Prepare the HEPES, NaCl, K2HPO4 and NH4Cl as a basal salt solution, adjusting the pH to 7.5-7.8 with NaOH before sterilisation. All other components are added from sterile stock solutions. |
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Nitrilotriacetic acid | 1.50 | g | ||
|
MgSO4 x 7 H2O | 3.00 | g | ||
|
MnSO4 x H2O | 0.50 | g | ||
|
NaCl | 1.00 | g | ||
|
FeSO4 x 7 H2O | 0.10 | g | ||
|
CoSO4 x 7 H2O | 0.18 | g | ||
|
CaCl2 x 2 H2O | 0.10 | g | ||
|
ZnSO4 x 7 H2O | 0.18 | g | ||
|
CuSO4 x 5 H2O | 0.01 | g | ||
|
AlK(SO4)2 x 12 H2O | 0.02 | g | ||
|
H3BO3 | 0.01 | g | ||
|
Na2MoO4 x 2 H2O | 0.01 | g | ||
|
NiCl2 x 6 H2O | 0.03 | g | ||
|
Na2SeO3 x 5 H2O | 0.30 | mg | ||
|
Na2WO4 x 2 H2O | 0.40 | mg | ||
|
Distilled water | 1000.00 | ml | ||
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH. |