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DSMZ

DSMZ 737 . DPM MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
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Medium for Parafrankia colletiae DSM 43829

This strain uses the standard medium without modifications.

Growth conditions: more than 4 weeks 28 °C

Main sol. 737 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1.00 g 1 7.348
MgSO4 x 7 H2O 0.10 g 0.1 0.406
CaCl2 x 2 H2O 0.01 g 0.01 0.068
Na-propionate 1.20 g 1.2 12.492
Chelated iron solution 1.80 ml - -
Trace element solution 1.00 ml - -
Distilled water 1000.00 ml - -
1 The pH of the final solution is adjusted to 6.8.

Chelated iron solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 5.54 g 5.54 19.927
Na2-EDTA 7.56 g 7.56 22.486
Distilled water 1000.00 ml - -

Trace element solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
CoCl2 0.025 g 0.025 0.193
CuSO4 x 5 H2O 0.080 g 0.08 0.32
H3BO3 2.860 g 2.86 46.248
MnCl2 x 4 H2O 1.810 g 1.81 9.146
Na2MoO4 x 2 H2O 0.025 g 0.025 0.103
ZnSO4 x 7 H2O 0.220 g 0.22 0.765
Distilled water 1000.000 ml - -

Metadata

Last modified: 20.09.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.8
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Associated strains

All strains for this medium: 7
Parafrankia colletiae DSM 43829

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na-propionate 1.2 12.492
KH2PO4 1 7.348
MgSO4 x 7 H2O 0.1 0.406
Na2-EDTA 0.014 0.04
CaCl2 x 2 H2O 0.01 0.068
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Cultivation metadata from 7 strains BacDive

pH 6 - 8 3 strains
phyla 1 7 strains
nutrition chemoorganotroph 2 strains
nutrition heterotroph 2 strains
oxygen aerobe 2 strains
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