737 .
DPM MEDIUM
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Selected strain: Frankia sp. DSM 44251
This strain uses the standard medium without modifications.
Growth conditions: 28 °C
Equipment needed:
Final pH:
6.8
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 1.00 | g | ||
|
|
MgSO4 x 7 H2O | 0.10 | g | ||
|
|
CaCl2 x 2 H2O | 0.01 | g | ||
|
|
Na-propionate | 1.20 | g | ||
|
|
Chelated iron solution | 1.80 | ml | ||
|
|
Trace element solution | 1.00 | ml | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 The pH of the final solution is adjusted to 6.8. | |||||
Chelated iron solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
FeSO4 x 7 H2O | 5.54 | g |
|
|
Na2-EDTA | 7.56 | g |
|
|
Distilled water | 1000.00 | ml |
Trace element solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
CoCl2 | 0.025 | g |
|
|
CuSO4 x 5 H2O | 0.080 | g |
|
|
H3BO3 | 2.860 | g |
|
|
MnCl2 x 4 H2O | 1.810 | g |
|
|
Na2MoO4 x 2 H2O | 0.025 | g |
|
|
ZnSO4 x 7 H2O | 0.220 | g |
|
|
Distilled water | 1000.000 | ml |