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DSMZ

DSMZ 743 . HYDROGENOBACTER ACIDOPHILUS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 743 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Sulfur 5.0 g 5 155.909
(NH4)2SO4 1.0 g 1 7.568
K2HPO4 1.0 g 1 5.741
NaCl 1.0 g 1 17.112
MgSO4 x 7 H2O 0.3 g 0.3 1.217
FeSO4 x 7 H2O 1.0 mg 1.0e-3 0.004
CaCl2 1.0 mg 1.0e-3 0.009
Trace element solution 0.5 ml - -
Distilled water 1000.0 ml - -
1 Adjust pH to 3.0 with HCl. Steam medium for 3 hours on each of 3 successive days. Incubate under a gas phase H2:O2:CO2 = 7:1:1 with an overpressure of one atmosphere at 65°C.

Trace element solution #

1000
Solution occurs in 2 other media (first defined in Medium 533).
Compound Amount Unit Conc. [g/L] Conc. [mM]
H2O
(deionized) 		1000 ml - -
MoO3 4 mg 0.004 0.028
ZnSO4 x 7 H2O 28 mg 0.028 0.097
CuSO4 x 5 H2O 2 mg 0.002 0.008
H3BO3 4 mg 0.004 0.065
MnSO4 x 5 H2O 4 mg 0.004 0.017
CoCl2 x 6 H2O 4 mg 0.004 0.031

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 3.0
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Gas composition

  • 77.7 % H2
  • 11.1 % CO2
  • 11.1 % O2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sulfur 5 155.909
NaCl 1 17.112
(NH4)2SO4 1 7.568
K2HPO4 1 5.741
MgSO4 x 7 H2O 0.3 1.217
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature thermophilic 1 strain