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DSMZ

DSMZ 748 . CENS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 748 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 0.9 g 0.9 5.167
KH2PO4 0.6 g 0.6 4.409
NH4Cl 1.0 g 1 18.695
EDTA 5.0 mg 0.005 0.017
MgSO4 x 7 H2O 0.2 g 0.2 0.811
CaCl2 x 2 H2O 75.0 mg 0.075 0.51
True Blue Trace Elements 1.0 ml - -
Chelated Iron solution 2.0 ml - -
Vitamin B12 20.0 µg 2.0e-5 1.5e-5
Biotin 15.0 µg 1.5e-5 6.1e-5
Na2S2O3 x 5 H2O 0.5 g 0.5 2.015
Soytone
(Difco 0436) 		4.0 g 4 -
Na-pyruvate
(filter-sterilized) 		2.2 g 2.2 19.992
Distilled water 1000.0 ml - -

True Blue Trace Elements #

250
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 2.5 g 10 34.219
MnCl2 x 4 H2O 0.2 g 0.8 4.042
H3BO3 0.1 g 0.4 6.468
Na2MoO4 x 2 H2O 0.1 g 0.4 1.653
ZnCl2 50.0 mg 0.2 1.467
NiCl2 x 6 H2O 50.0 mg 0.2 1.543
CoCl2 x 2 H2O 20.0 mg 0.08 0.616
CuCl2 x 2 H2O 10.0 mg 0.04 0.235
Na2SeO3 5.0 mg 0.02 0.116
NaVO3 x n H2O
(meta) 		5.0 mg 0.02 0.143
Distilled water 250.0 ml - -
1 Dissolve chemicals one at a time in approximately 200.0 ml distilled water. Bring final volume to 250.0 ml with additional water.

Chelated Iron solution #

900
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeCl2 x 4 H2O 1 g 1.111 5.589
EDTA 2 g 2.222 7.604
HCl
(conc.) 		3 ml - -
Distilled water 900 ml - -

Metadata

Last modified: 24.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Soytone 4 -
Na-pyruvate 2.2 19.992
NH4Cl 1 18.695
K2HPO4 0.9 5.167
KH2PO4 0.6 4.409
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain
temperature thermophilic 1 strain