751
751 .
SC MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 751 #
ml
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Corn meal agar | 17.0 | g | 17 | - | |
| Soy peptone | 8.0 | g | 8 | - | |
| K2HPO4 | 1.0 | g | 1 | 5.741 | |
| KH2PO4 | 1.0 | g | 1 | 7.348 | |
| MgSO4 x 7 H2O | 0.2 | g | 0.2 | 0.811 | |
|
Hemin
(0.1% in 0.05N NaOH) |
15.0 | ml | 0.015 | 0.023 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 6.6. Autoclave, cool to 50°C and add the following filter-sterilized components: | |||||
|
Bovine serum albumin
, fraction V
(20%) |
10.0 | ml | 2 | - | |
|
Glucose
(10%) |
5.0 | ml | 0.5 | 2.775 | |
|
Cysteine
(free base, 10%) |
10.0 | ml | 1 | 8.254 | |
| 2 For liquid medium, omit corn meal agar and change the phosphate concentration to KH2PO4, 1.5 g and K2HPO4, 0.5 g. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.6 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 2 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Corn meal agar | 17 | - |
| Soy peptone | 8 | - |
| Bovine serum albumin | 2 | - |
| Cysteine | 1 | 8.254 |
| KH2PO4 | 1 | 7.348 |
| Show more | ||
