752 .
HALOMONAS PANTELLERIENSE
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Selected strain: Halomonas pantelleriensis DSM 9661
This strain uses the standard medium without modifications.
Growth conditions: 30 °C
Equipment needed:
Autoclave
Final pH:
9.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Yeast extract | 10 | g | ||
|
|
Na2-citrate | 3 | g | ||
|
|
KCl | 2 | g | ||
|
|
MgSO4 x 7 H2O | 1 | g | ||
|
|
NaCl | 100 | g | ||
|
|
Na2CO3 | 3 | g | ||
|
|
MnCl2 x 4 H2O
(0.36g/l) |
1 | ml | ||
|
|
FeSO4 x 7 H2O
(50g/l) |
1 | ml | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Liquid medium may be prepared by adding all components together. Agar (2%) medium should be prepared without Na2CO3 and sterilised by autoclaving. Add 20-30 ml of a sterile 10% Na2CO3 solution to the autoclaved medium containing agar so that the final pH is 9.0. | |||||