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76. GOTTSCHALKIA MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.67 g 0.665 11.847
2 0.91 g 0.903 5.183
3 2.00 g 1.984 11.803
4 0.25 g 0.248 1.006
5 15.00 mg 0.015 0.101
6
(0.1% w/v in 0.1 N H2SO4)
6.00 ml 0.006 0.021
7 Trace element solution SL-10 1.00 ml - -
8 Selenite-tungstate solution 1.00 ml - -
9 1.00 g 0.992 -
10
(0.1% w/v)
0.50 ml 5.0e-4 0.002
11 1.50 g 1.488 14.04
12 0.50 g 0.496 4.347
13 1000.00 ml 990.285 54969.1
Instructions:
  1. First dissolve KOH and K2HPO4 in water, then add uric acid and boil until the acid is dissolved. Cool medium to room temperature under 100% N2 gas atmosphere and add all other compounds, except carbonate and thioglycolate. Dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave for 15 min at 121°C. Then add carbonate (filter-sterilized stock solution prepared under 80% N2 and 20% CO2 gas atmosphere) and thioglycolate (stock solution, autoclaved separately under 100% N2 gas). Adjust pH of complete medium to 7.0 - 7.5, if necessary.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.3
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 14
Strains with modifications: 14

Gas composition

  • 100 % N2

Light

14 strains need light for growth. Select a strain for more details.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 992.256 55078.5
Uric acid 1.984 11.803
Na2CO3 1.488 14.04
Yeast extract 0.992 -
K2HPO4 0.903 5.183
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Cultivation metadata from 14 strains BacDive

pH 6 - 8 3 strains
pH 8 - 10 2 strains
pH 10 - 14 1 strain
phyla 1 14 strains
nutrition chemoorganotroph 2 strains
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