DSMZ 761 . HALOTHERMOTHRIX ORENII

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.0 g 1 18.695
K2HPO4 0.3 g 0.3 1.722
KH2PO4 0.3 g 0.3 2.205
MgCl2 x 6 H2O 2.0 g 2 9.838
CaCl2 x 2 H2O 0.2 g 0.2 1.36
KCl 4.0 g 4 53.655
Na-acetate 1.0 g 1 12.19
Glucose 10.0 g 10 55.506
Trypticase 0.5 g 0.5 -
NaCl 100.0 g 100 1711.16
Resazurin 1.0 mg 1.0e-3 0.004
Modified Wolin's mineral solution II 1.0 ml - -
Distilled water 1000.0 ml - -
1 Prepare the medium and boil, cool under N2:CO2 (80:20 v/v). Dispense medium into Hungate tubes or serum bottles under N2:CO2 (80:20 v/v), autoclave. To the sterile medium add from sterile stock solutions 2% Na2S x 9 H2O (0.1ml/10ml), 10% NaHCO3 (0.5ml/10ml), and 0.2% sodium dithionite (0.05ml/10ml). Final pH 7.0
Solution occurs in 22 other media (first defined in Medium 700).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.500 g - -
MgSO4 x 7 H2O 3.000 g - -
MnSO4 x H2O 0.500 g - -
NaCl 1.000 g - -
FeSO4 x 7 H2O 0.100 g - -
CoSO4 x 7 H2O 0.180 g - -
CaCl2 x 2 H2O 0.100 g - -
ZnSO4 x 7 H2O 0.180 g - -
CuSO4 x 5 H2O 0.010 g - -
AlK(SO4)2 x 12 H2O 0.020 g - -
H3BO3 0.010 g - -
Na2MoO4 x 2 H2O 0.010 g - -
NiCl2 x 6 H2O 0.025 g - -
Na2SeO3 x 5 H2O 0.300 mg - -
Distilled water 1000.000 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Source: DSMZ
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Hungate tubes

Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Glucose 10 55.506
KCl 4 53.655
MgCl2 x 6 H2O 2 9.838
NH4Cl 1 18.695
Na-acetate 1 12.19
Show more