Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
NH4Cl | 1.0 | g | ||
|
K2HPO4 | 0.3 | g | ||
|
KH2PO4 | 0.3 | g | ||
|
MgCl2 x 6 H2O | 2.0 | g | ||
|
CaCl2 x 2 H2O | 0.2 | g | ||
|
KCl | 4.0 | g | ||
|
Na-acetate | 1.0 | g | ||
|
Glucose | 10.0 | g | ||
|
Trypticase | 0.5 | g | ||
|
NaCl | 100.0 | g | ||
|
Resazurin | 1.0 | mg | ||
|
Modified Wolin's mineral solution II | 1.0 | ml | ||
|
Distilled water | 1000.0 | ml | ||
1 Prepare the medium and boil, cool under N2:CO2 (80:20 v/v). Dispense medium into Hungate tubes or serum bottles under N2:CO2 (80:20 v/v), autoclave. To the sterile medium add from sterile stock solutions 2% Na2S x 9 H2O (0.1ml/10ml), 10% NaHCO3 (0.5ml/10ml), and 0.2% sodium dithionite (0.05ml/10ml). Final pH 7.0 |
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Nitrilotriacetic acid | 1.500 | g | ||
|
MgSO4 x 7 H2O | 3.000 | g | ||
|
MnSO4 x H2O | 0.500 | g | ||
|
NaCl | 1.000 | g | ||
|
FeSO4 x 7 H2O | 0.100 | g | ||
|
CoSO4 x 7 H2O | 0.180 | g | ||
|
CaCl2 x 2 H2O | 0.100 | g | ||
|
ZnSO4 x 7 H2O | 0.180 | g | ||
|
CuSO4 x 5 H2O | 0.010 | g | ||
|
AlK(SO4)2 x 12 H2O | 0.020 | g | ||
|
H3BO3 | 0.010 | g | ||
|
Na2MoO4 x 2 H2O | 0.010 | g | ||
|
NiCl2 x 6 H2O | 0.025 | g | ||
|
Na2SeO3 x 5 H2O | 0.300 | mg | ||
|
Distilled water | 1000.000 | ml | ||
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH. |