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DSMZ

DSMZ 765 . ISOSPHAERA PALLIDA MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 765 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 250 ml - -
Distilled water 650 ml - -
Solution B 50 ml - -
1 Prepare the medium by mixing 250 ml of solution A (without vitamins added) with 650 ml distilled water. Gas with air:CO2 (95:5 v/v), and fill the medium into serum bottles so that the gas to liquid ratio is about 5:1 (v/v). Gas the serum bottles with air:CO2 (95:5 v/v), seal the bottles as for anaerobic work and autoclave. To the cooled medium add (using a syringe) 10ml NaHCO3/ 90ml medium and 0.5ml sterile vitamin solution to 100ml of the resulting medium. Final pH 7.9.

Solution A #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 1.000 g 1 7.568
KH2PO4 0.300 g 0.3 2.205
MgSO4 x 7 H2O 2.000 g 2 8.114
CaCl2 x 2 H2O 0.200 g 0.2 1.36
KCl 4.000 g 4 53.655
NaCl 1.000 g 1 17.112
FeCl3 0.292 mg 2.9e-4 0.002
Glucose 0.250 g 0.25 1.388
Casamino acids 0.250 g 0.25 -
Vitamin solution 1.000 ml - -
Trace element solution SL-7a 1.000 ml - -
Distilled water 1000.000 ml - -

Trace element solution SL-7a #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		1 ml 0.25 6.857
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 60 mg 0.06 0.97
ZnCl2 70 mg 0.07 0.514
CoCl2 x 6 H2O 200 mg 0.2 1.54
NiCl2 x 6 H2O 20 mg 0.02 0.154
Na2MoO4 x 2 H2O 40 mg 0.04 0.165
CuCl2 x 2 H2O 20 mg 0.02 0.117
Distilled water 999 ml - -

Solution B #

50
Equipment needed: Anaerobic workbench Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 2.1 g 42 499.958
Distilled water 50.0 ml - -
1 Fill into 100 ml serum bottle, gas with CO2 , seal as for anaerobic work and autoclave. Cool before adding to the final medium.

Vitamin solution #

100
Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 0.1 mg 1.0e-3 7.4e-4
Nicotinic acid 20.0 mg 0.2 1.625
Thiamine-HCl x 2 H2O 10.0 mg 0.1 0.268
p-Aminobenzoic acid 0.2 mg 0.002 0.015
Distilled water 100.0 ml - -
1 Filter sterilise.

Metadata

Last modified: 24.02.22
Source: DSMZ
Medium type: Complex medium
Final pH: 7.9
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 2 23.808
KCl 0.952 12.775
MgSO4 x 7 H2O 0.476 1.932
NaCl 0.238 4.074
(NH4)2SO4 0.238 1.802
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Cultivation metadata from 0 strains BacDive

phyla 1 1 strain