This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
(NH4)2SO4 | 0.50 | g | 0.5 | 3.784 | |
NaHCO3 | 0.20 | g | 0.2 | 2.381 | |
KH2PO4 | 0.50 | g | 0.5 | 3.674 | |
Na2WO4 x 2 H2O | 0.15 | mg | 1.5e-4 | 4.5e-4 | |
Na2SeO4 | 0.15 | mg | 1.5e-4 | 7.9e-4 | |
(NH4)2Ni(SO4)2 | 0.30 | mg | 3.0e-4 | 7.6e-4 | |
Chitin stock solution | 4.00 | g | 4 | - | |
Na2S x 9 H2O | 0.50 | g | 0.5 | 2.082 | |
Modified Wolin's mineral solution II | 15.00 | ml | - | - | |
Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
Artifical sea water
(synthetic) |
485.00 | ml | - | - | |
Distilled water | 500.00 | ml | - | - | |
1 Prepare the medium without Na2S x 9 H2O and NaHCO3, boil and cool under N2. Add the NaHCO3 to the cooled medium and adjust the pH to 6.7. Dispense into Hungate tubes or serum bottles under N2 and autoclave. Reduce the medium before use from a sterile stock solution of neutralised Na2S x 9 H2O. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Chitin | 20 | g | 16.667 | - | |
HCl
(37 %) |
200 | ml | 74 | 2029.62 | |
Distilled water | 1000 | ml | - | - | |
1 The chitin solution is prepared as follows: mix 20 g chitin (practical grade from crab shells) with 200 ml of 37% HCl (pre-cooled to 4°C) and stir for 1 hr at 4°C. Pour the suspension into 1 litre of distilled water (pre-cooled to 4°C) and filter through filter paper (Schleicher and Schüll Nr. 311853). Wash the residue five times with 500 ml distilled water and resuspend in 1 litre of distilled water. Neutralise the suspension with 10 ml of 5M KOH (final pH 6.5). Filter and wash with 3 litres of distilled water to remove KCl. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
NaCl | 23.47 | g | 23.47 | 401.608 |
Na2SO4 | 3.92 | g | 3.92 | 27.598 |
MgCl2 x 6 H2O | 10.64 | g | 10.64 | 52.336 |
CaCl2 | 1.10 | g | 1.1 | 9.912 |
NaHCO3 | 192.00 | mg | 0.192 | 2.286 |
KCl | 664.00 | mg | 0.664 | 8.907 |
KBr | 96.00 | mg | 0.096 | 0.807 |
H3BO3 | 26.00 | mg | 0.026 | 0.42 |
SrCl2 | 24.00 | mg | 0.024 | 0.151 |
NaF | 3.00 | mg | 0.003 | 0.071 |
Distilled water | 1000.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid | 1.500 | g | 1.5 | 7.848 | |
MgSO4 x 7 H2O | 3.000 | g | 3 | 12.172 | |
MnSO4 x H2O | 0.500 | g | 0.5 | 2.958 | |
NaCl | 1.000 | g | 1 | 17.112 | |
FeSO4 x 7 H2O | 0.100 | g | 0.1 | 0.36 | |
CoSO4 x 7 H2O | 0.180 | g | 0.18 | 1.161 | |
CaCl2 x 2 H2O | 0.100 | g | 0.1 | 0.68 | |
ZnSO4 x 7 H2O | 0.180 | g | 0.18 | 0.626 | |
CuSO4 x 5 H2O | 0.010 | g | 0.01 | 0.04 | |
AlK(SO4)2 x 12 H2O | 0.020 | g | 0.02 | 0.042 | |
H3BO3 | 0.010 | g | 0.01 | 0.162 | |
Na2MoO4 x 2 H2O | 0.010 | g | 0.01 | 0.041 | |
NiCl2 x 6 H2O | 0.025 | g | 0.025 | 0.193 | |
Na2SeO3 x 5 H2O | 0.300 | mg | 3.0e-4 | 0.001 | |
Distilled water | 1000.000 | ml | - | - | |
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH. |
Last modified: | 22.02.22 |
Source: | DSMZ |
Taxonomic range: | Archaea |
Medium type: | Defined medium |
Final pH: | 6.7 |
Equipment needed: | Autoclave, Filter, Hungate tubes |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 11.383 | 194.78 |
MgCl2 x 6 H2O | 5.16 | 25.383 |
Na2SO4 | 1.901 | 13.385 |
CaCl2 | 0.534 | 4.807 |
(NH4)2SO4 | 0.5 | 3.784 |
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