DSMZ 768 . M17 MEDIUM FOR FILOMICROBIUM FUSIFORME

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-acetate 1 g 1 12.19
KNO3 1 g 1 9.891
Hutner's salts 20 ml - -
Vitamin solution (double conc.) 5 ml - -
Artificial sea water 500 ml - -
Distilled water 475 ml - -
1 Final pH 7.2.
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 23.48 g 23.48 401.78
Na2SO4 3.92 g 3.92 27.598
NaHCO3 0.19 g 0.19 2.262
KCl 0.66 g 0.66 8.853
KBr 0.10 g 0.1 0.84
MgCl2 x 6 H2O 5.00 g 5 24.594
CaCl2 x 2 H2O 1.10 g 1.1 7.482
H3BO3 0.03 g 0.03 0.485
SrCl2 0.02 g 0.02 0.126
NaF 3.00 mg 0.003 0.071
Distilled water 1000.00 ml - -
1 Add NaHCO3 from a filter-sterilized stock solution when the medium has cooled.
Solution occurs in 24 other media (first defined in Medium 590).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA)
10.000 g 10 52.318
MgSO4 x 7 H2O 29.700 g 29.7 120.499
CaCl2 x 2 H2O 3.335 g 3.335 22.685
(NH4)6Mo7O24 x 4 H2O 9.250 mg 0.009 -
FeSO4 x 7 H2O 99.000 mg 0.099 0.356
Metall salt sol. 44 50.000 ml - -
Distilled water 950.000 ml - -
1 Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 7.3 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4.
Solution occurs in 18 other media (first defined in Medium 590).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-EDTA 250.0 mg 0.25 0.744
ZnSO4 x 7 H2O 1095.0 mg 1.095 3.808
FeSO4 x 7 H2O 500.0 mg 0.5 1.798
MnSO4 x H2O 154.0 mg 0.154 0.911
CuSO4 x 5 H2O 39.2 mg 0.039 0.157
Co(NO3)2 x 6 H2O 24.8 mg 0.025 0.085
Na2B4O7 x 10 H2O 17.7 mg 0.018 -
Distilled water 1000.0 ml - -
1 Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.
Solution occurs in 6 other media (first defined in Medium 621).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 4.0 mg 0.004 0.016
Folic acid 4.0 mg 0.004 0.009
Pyridoxine hydrochloride 20.0 mg 0.02 0.097
Riboflavine 10.0 mg 0.01 0.027
Thiamine-HCl x 2 H2O 10.0 mg 0.01 0.027
Nicotinamide 10.0 mg 0.01 0.082
D-Calcium pantothenate 10.0 mg 0.01 -
Vitamin B12 0.2 mg 2.0e-4 1.5e-4
p-Aminobenzoic acid 10.0 mg 0.01 0.073
Distilled water 1000.0 ml - -
1 Store in the dark and cold (5°C).

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.2
Equipment needed: Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 11.74 200.89
MgCl2 x 6 H2O 2.5 12.297
Na2SO4 1.96 13.799
Na-acetate 1 12.19
KNO3 1 9.891
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain