This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na-acetate | 1 | g | 1 | 12.19 | |
KNO3 | 1 | g | 1 | 9.891 | |
Hutner's salts | 20 | ml | - | - | |
Vitamin solution (double conc.) | 5 | ml | - | - | |
Artificial sea water | 500 | ml | - | - | |
Distilled water | 475 | ml | - | - | |
1 Final pH 7.2. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 23.48 | g | 23.48 | 401.78 | |
Na2SO4 | 3.92 | g | 3.92 | 27.598 | |
NaHCO3 | 0.19 | g | 0.19 | 2.262 | |
KCl | 0.66 | g | 0.66 | 8.853 | |
KBr | 0.10 | g | 0.1 | 0.84 | |
MgCl2 x 6 H2O | 5.00 | g | 5 | 24.594 | |
CaCl2 x 2 H2O | 1.10 | g | 1.1 | 7.482 | |
H3BO3 | 0.03 | g | 0.03 | 0.485 | |
SrCl2 | 0.02 | g | 0.02 | 0.126 | |
NaF | 3.00 | mg | 0.003 | 0.071 | |
Distilled water | 1000.00 | ml | - | - | |
1 Add NaHCO3 from a filter-sterilized stock solution when the medium has cooled. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid
(NTA) |
10.000 | g | 10 | 52.318 | |
MgSO4 x 7 H2O | 29.700 | g | 29.7 | 120.499 | |
CaCl2 x 2 H2O | 3.335 | g | 3.335 | 22.685 | |
(NH4)6Mo7O24 x 4 H2O | 9.250 | mg | 0.009 | 0.007 | |
FeSO4 x 7 H2O | 99.000 | mg | 0.099 | 0.356 | |
"Metals 44" | 50.000 | ml | - | - | |
Distilled water | 950.000 | ml | - | - | |
1 Dissolve the nitrilotriacetic acid. | |||||
2 Adjust pH to 7.0 with KOH (~7.3 g). | |||||
3 Dissolve other salts separately and combine. | |||||
4 Adjust pH to 6.8 with NaOH or H2SO4. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na-EDTA | 250.0 | mg | 0.25 | 0.744 | |
ZnSO4 x 7 H2O | 1095.0 | mg | 1.095 | 3.808 | |
FeSO4 x 7 H2O | 500.0 | mg | 0.5 | 1.798 | |
MnSO4 x H2O | 154.0 | mg | 0.154 | 0.911 | |
CuSO4 x 5 H2O | 39.2 | mg | 0.039 | 0.157 | |
Co(NO3)2 x 6 H2O | 24.8 | mg | 0.025 | 0.085 | |
Na2B4O7 x 10 H2O | 17.7 | mg | 0.018 | - | |
Distilled water | 1000.0 | ml | - | - | |
1 Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Biotin | 4.0 | mg | 0.004 | 0.016 | |
Folic acid | 4.0 | mg | 0.004 | 0.009 | |
Pyridoxine hydrochloride | 20.0 | mg | 0.02 | 0.097 | |
Riboflavine | 10.0 | mg | 0.01 | 0.027 | |
Thiamine-HCl x 2 H2O | 10.0 | mg | 0.01 | 0.027 | |
Nicotinamide | 10.0 | mg | 0.01 | 0.082 | |
D-Calcium pantothenate | 10.0 | mg | 0.01 | - | |
Vitamin B12 | 0.2 | mg | 2.0e-4 | 1.5e-4 | |
p-Aminobenzoic acid | 10.0 | mg | 0.01 | 0.073 | |
Distilled water | 1000.0 | ml | - | - | |
1 Store in the dark and cold (5°C). |
Last modified: | 22.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | 7.2 |
Equipment needed: | Filter |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 11.74 | 200.89 |
MgCl2 x 6 H2O | 2.5 | 12.297 |
Na2SO4 | 1.96 | 13.799 |
Na-acetate | 1 | 12.19 |
KNO3 | 1 | 9.891 |
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