76
76 .
GOTTSCHALKIA MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Clostridium cylindrosporum DSM 605
This strain uses the standard medium without modifications.
Growth conditions: anaerobic
37 °C
Main sol. 76 #
ml
Equipment needed: Hungate tubes
Filter
Gassing station
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KOH | 0.67 | g | 0.67 | 11.942 | |
| K2HPO4 | 0.91 | g | 0.91 | 5.225 | |
| Uric acid | 2.00 | g | 2 | 11.897 | |
| MgSO4 x 7 H2O | 0.25 | g | 0.25 | 1.014 | |
| CaCl2 x 2 H2O | 15.00 | mg | 0.015 | 0.102 | |
|
FeSO4 x 7 H2O
(0.1% w/v in 0.1 N H2SO4) |
6.00 | ml | 0.006 | 0.022 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
| Yeast extract | 1.00 | g | 1 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
| Na2CO3 | 1.50 | g | 1.5 | 14.153 | |
| Na-thioglycolate | 0.50 | g | 0.5 | 4.382 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 First dissolve KOH and K2HPO4 in water, then add uric acid and boil until the acid is dissolved. Cool medium to room temperature under 100% N2 gas atmosphere and add all other compounds, except carbonate and thioglycolate. Dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave for 15 min at 121°C. Then add carbonate (filter-sterilized stock solution prepared under 80% N2 and 20% CO2 gas atmosphere) and thioglycolate (stock solution, autoclaved separately under 100% N2 gas). Adjust pH of complete medium to 7.0 - 7.5, if necessary. | |||||
Solution occurs in 265
other media (first defined in Medium 320).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 990.0 | ml | - | - | |
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Solution occurs in 178
other media (first defined in Medium 385).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaOH | 0.5 | g | 0.5 | 12.501 |
| Na2SeO3 x 5 H2O | 3.0 | mg | 0.003 | 0.011 |
| Na2WO4 x 2 H2O | 4.0 | mg | 0.004 | 0.012 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 - 7.5 |
| Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 14 |
Clostridium cylindrosporum DSM 605
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Uric acid | 2 | 11.897 |
| Na2CO3 | 1.5 | 14.153 |
| Yeast extract | 1 | - |
| K2HPO4 | 0.91 | 5.225 |
| KOH | 0.67 | 11.942 |
| Show more | ||
Cultivation metadata from 14 strains
| pH | 6 - 8 | 3 strains |
| pH | 8 - 10 | 2 strains |
| phyla | 1 | 14 strains |
| nutrition | chemoorganotroph | 2 strains |
| oxygen | anaerobe | 14 strains |
| Show more | ||