770 .
SULFOPHOBOCOCCUS ZILLIGII MEDIUM
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Equipment needed:
Autoclave
Filter
Gassing station
Serum bottle
Final gas composition:
100 % N2
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Final pH:
7.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
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Solution A | 970 | ml | ||
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Solution B | 10 | ml | ||
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Solution C | 10 | ml | ||
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Solution D | 5 | ml | ||
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Solution E | 10 | ml | ||
| 1 Dissolve ingredients of solution A except carbonate, sparge with 100% N2 gas for 30 - 45 min to make it anoxic, then add carbonate and adjust pH to 9.0 - 9.3. Dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials (e.g., 30 ml medium per 100 ml bottle) and autoclave. Solutions B and C are autoclaved separately under 100% N2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas and sterilized by filtration. To complete the medium add appropriate amounts of solutions B to E to the sterile solution A in the sequence as indicated. Adjust pH of complete medium to 7.5. | |||||
| 2 Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth at the beginning. | |||||
| 3 For transfers use 10% (v/v) inoculum. | |||||
Solution A #
970
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Na2-EDTA | 32.0 | mg |
|
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CaCl2 x 2 H2O | 66.0 | mg |
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MgSO4 x 7 H2O | 31.0 | mg |
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KCl | 31.0 | mg |
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ZnCl2
(0.1% w/v) |
2.1 | ml |
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MnSO4 x H2O
(0.1% w/v) |
2.3 | ml |
|
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Na2B4O7 x 10 H2O
(0.1% w/v) |
1.8 | ml |
|
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Glycine | 1.5 | g |
|
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Sodium resazurin
(0.1% w/v) |
0.5 | ml |
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Na2CO3 | 230.0 | mg |
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Distilled water | 965.0 | ml |