DSMZ 772 . PARACOCCUS ALCALIPHILUS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Selected strain: Paracoccus alcaliphilus DSM 8512

This strain uses the standard medium without modifications.

Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 3.0 g 3 22.703
KH2PO4 1.4 g 1.4 10.288
Na2HPO4 3.0 g 3 21.133
MgSO4 x 7 H2O 0.2 g 0.2 0.811
Fe(III) citrate 30.0 mg 0.03 0.122
CaCl2 x 2 H2O 30.0 mg 0.03 0.204
MnCl2 x 4 H2O 5.0 mg 0.005 0.025
ZnSO4 x 7 H2O 5.0 mg 0.005 0.017
CuSO4 x 2 H2O 0.5 mg 5.0e-4 0.003
Thiamine-HCl x 2 H2O 0.4 mg 4.0e-4 0.001
Distilled water 1000.0 ml - -
Methanol
(filter-sterilized)
10.0 ml 7.92 247.176
1 Thiamine hydrochloride may be replaced by 2 g/l yeast extract. The pH of the medium is adjusted to pH 9.0 with filter sterilised 10% Na2CO3. 10 ml sterile methanol (filter sterilised, or autoclaved in crimp top sealed bottles) is added to the sterilised medium.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 9.0
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1
Paracoccus alcaliphilus DSM 8512

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Methanol 7.92 247.176
(NH4)2SO4 3 22.703
Na2HPO4 3 21.133
KH2PO4 1.4 10.288
MgSO4 x 7 H2O 0.2 0.811
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain