772 .
PARACOCCUS ALCALIPHILUS MEDIUM
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Selected strain: Paracoccus alcaliphilus DSM 8512
This strain uses the standard medium without modifications.
Growth conditions: 30 °C
Equipment needed:
Autoclave
Filter
Final pH:
9.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
(NH4)2SO4 | 3.0 | g | ||
|
|
KH2PO4 | 1.4 | g | ||
|
|
Na2HPO4 | 3.0 | g | ||
|
|
MgSO4 x 7 H2O | 0.2 | g | ||
|
|
Fe(III) citrate | 30.0 | mg | ||
|
|
CaCl2 x 2 H2O | 30.0 | mg | ||
|
|
MnCl2 x 4 H2O | 5.0 | mg | ||
|
|
ZnSO4 x 7 H2O | 5.0 | mg | ||
|
|
CuSO4 x 2 H2O | 0.5 | mg | ||
|
|
Thiamine-HCl x 2 H2O | 0.4 | mg | ||
|
|
Distilled water | 1000.0 | ml | ||
|
|
Methanol
(filter-sterilized) |
10.0 | ml | ||
| 1 Thiamine hydrochloride may be replaced by 2 g/l yeast extract. The pH of the medium is adjusted to pH 9.0 with filter sterilised 10% Na2CO3. 10 ml sterile methanol (filter sterilised, or autoclaved in crimp top sealed bottles) is added to the sterilised medium. | |||||