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DSMZ

DSMZ 773 . PARACOCCUS KOCURII MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Paracoccus kocurii DSM 8536

This strain uses the standard medium without modifications.

Growth conditions: 28 °C

Main sol. 773 #

ml
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaH2PO4 x 2 H2O 1.71 g 1.71 14.253
K2HPO4 1.41 g 1.41 8.095
(NH4)2SO4 1.00 g 1 7.568
Tetramethyl ammonium chloride 1.00 g 1 -
Thiamine-HCl x 2 H2O 0.50 mg 5.0e-4 0.001
Hutner's salts 20.00 ml - -
Distilled water 1000.00 ml - -
1 Final pH 6.8-7.0
2 The tetramethyl ammonium chloride and thiamine hydrochloride are added from filter sterilised stock solutions.

Hutner's salts #

1000
Solution occurs in 24 other media (first defined in Medium 590).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA) 		10.000 g 10 52.318
MgSO4 x 7 H2O 29.700 g 29.7 120.499
CaCl2 x 2 H2O 3.335 g 3.335 22.685
(NH4)6Mo7O24 x 4 H2O 9.250 mg 0.009 0.007
FeSO4 x 7 H2O 99.000 mg 0.099 0.356
"Metals 44" 50.000 ml - -
Distilled water 950.000 ml - -
1 Dissolve the nitrilotriacetic acid.
2 Adjust pH to 7.0 with KOH (~7.3 g).
3 Dissolve other salts separately and combine.
4 Adjust pH to 6.8 with NaOH or H2SO4.

"Metals 44" #

1000
Solution occurs in 34 other media (first defined in Medium 590).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-EDTA 250.0 mg 0.25 0.744
ZnSO4 x 7 H2O 1095.0 mg 1.095 3.808
FeSO4 x 7 H2O 500.0 mg 0.5 1.798
MnSO4 x H2O 154.0 mg 0.154 0.911
CuSO4 x 5 H2O 39.2 mg 0.039 0.157
Co(NO3)2 x 6 H2O 24.8 mg 0.025 0.085
Na2B4O7 x 10 H2O 17.7 mg 0.018 -
Distilled water 1000.0 ml - -
1 Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.

Metadata

Last modified: 24.03.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.8 - 7.0
Equipment needed: Filter
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Associated strains

All strains for this medium: 1
Paracoccus kocurii DSM 8536

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaH2PO4 x 2 H2O 1.71 14.253
K2HPO4 1.41 8.095
(NH4)2SO4 1 7.568
Tetramethyl ammonium chloride 1 -
MgSO4 x 7 H2O 0.594 2.41
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain