DSMZ 775 . R - CW MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

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Selected strain: Lentilactobacillus parakefiri DSM 10551

This strain uses the standard medium without modifications.

Equipment needed: Autoclave Wheaton tubes Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trypticase 5.0 g 5 -
Tryptone 5.0 g 5 70.343
Yeast extract 5.0 g 5 -
KH2PO4 5.0 g 5 36.742
Na-acetate 5.0 g 5 60.951
(NH4)2 citrate 2.0 g 2 -
MgSO4 x 7 H2O 0.5 g 0.5 2.029
MnSO4 x H2O 0.2 g 0.2 1.183
Tween 80 1.0 ml - -
Cheese whey 1000.0 ml - -
1 Adjust pH to 5.5 and distribute into screw-capped Wheaton tubes or Hungate tubes.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 5.5
Equipment needed: Autoclave, Hungate tubes, Wheaton tubes
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Associated strains

All strains for this medium: 2
Lentilactobacillus parakefiri DSM 10551

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Tryptone 5 70.343
Na-acetate 5 60.951
KH2PO4 5 36.742
Yeast extract 5 -
Trypticase 5 -
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
oxygen anaerobe 2 strains
temperature mesophilic 2 strains