775 .
R - CW MEDIUM
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Selected strain: Lentilactobacillus parakefiri DSM 10551
This strain uses the standard medium without modifications.
Growth conditions: 30 °C
Equipment needed:
Autoclave
Hungate tubes
Wheaton tubes
Final pH:
5.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Trypticase | 5.0 | g | ||
|
|
Tryptone | 5.0 | g | ||
|
|
Yeast extract | 5.0 | g | ||
|
|
KH2PO4 | 5.0 | g | ||
|
|
Na-acetate | 5.0 | g | ||
|
|
(NH4)2 citrate | 2.0 | g | ||
|
|
MgSO4 x 7 H2O | 0.5 | g | ||
|
|
MnSO4 x H2O | 0.2 | g | ||
|
|
Tween 80 | 1.0 | ml | ||
|
|
Cheese whey | 1000.0 | ml | ||
| 1 Adjust pH to 5.5 and distribute into screw-capped Wheaton tubes or Hungate tubes. | |||||