DSMZ 779 . CAPNOCYTOPHAGA II MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

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Selected strain: Capnocytophaga canimorsus DSM 19204

This strain uses the standard medium without modifications.

Equipment needed: Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Lab-Lemco beef extract
(Oxoid)
2.4 g 2.4 -
Proteose peptone no. 3
(Difco)
10.0 g 10 -
Yeast extract
(Difco, Bacto)
5.0 g 5 -
Na2HPO4 4.0 g 4 28.177
Glucose 1.5 g 1.5 8.326
Starch
(soluble)
0.5 g 0.5 -
L-Cysteine HCl x H2O 0.5 g 0.5 2.847
Agar , if necessary 15.0 g 15 44.599
Horse blood 50.0 ml - -
Distilled water 1000.0 ml - -
1 Adjust pH to 7.6 - 7.8.
2 Add the horse blood to the heat sterilized medium after being cooled to 50°C. Incubate under air plus 5% CO2 or anaerobically under 95% N2 plus 5% CO2.

Metadata

Last modified: 19.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.6 - 7.8
Equipment needed: Autoclave, Gassing station
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Gas composition

  • 95 % N2
  • 5 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 3
Capnocytophaga canimorsus DSM 19204

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Proteose peptone no. 3 10 -
Yeast extract 5 -
Na2HPO4 4 28.177
Lab-Lemco beef extract 2.4 -
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Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
oxygen facultative anaerobe 2 strains
oxygen microaerophile 1 strain
temperature mesophilic 3 strains