This strain uses the standard medium without modifications.
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Lab-Lemco beef extract
(Oxoid) |
2.4 | g | ||
|
Proteose peptone no. 3
(Difco) |
10.0 | g | ||
|
Yeast extract
(Difco, Bacto) |
5.0 | g | ||
|
Na2HPO4 | 4.0 | g | ||
|
Glucose | 1.5 | g | ||
|
Starch
(soluble) |
0.5 | g | ||
|
L-Cysteine HCl x H2O | 0.5 | g | ||
|
Agar , if necessary | 15.0 | g | ||
|
Horse blood | 50.0 | ml | ||
|
Distilled water | 1000.0 | ml | ||
1 Adjust pH to 7.6 - 7.8. | |||||
2 Add the horse blood to the heat sterilized medium after being cooled to 50°C. Incubate under air plus 5% CO2 or anaerobically under 95% N2 plus 5% CO2. |