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78. CHOPPED MEAT MEDIUM

Culture medium recipe

Vol.
×

M 78 #

ml
Solution occurs in 3 other media (first defined in medium 78 )
Equipment needed: Hungate tubes Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(fat free)
500.0 g 500 -
2 1000.0 ml 998.207 55408.8
3
(1 N)
25.0 ml 53.25 1331.35
4 30.0 g 30 -
5 5.0 g 5 -
6 5.0 g 5 28.707
7 1.0 mg 1.0e-3 0.004
11 0.5 g/l 0.5 3.172
12
optional
15.0 g/l 15 -
13
(5% w/v)
optional
1.0 g/l 1 11.904
Instructions:
  1. Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water and NaOH, then boil for 15 min with stirring. Cool to room temperature, skim fat off surface, and filter, retaining both meat particles and filtrate. To the filtrate add water to a final volume of 1000 ml, and then add: #4-7
  2. To make medium anoxic bring it to a boil, cool under 100% N2 gas atmosphere, add 0.5 g/l L-cysteine hydrochloride and adjust pH to 7.0. Dispense under 100% N2 gas atmosphere by filling 7 ml medium into anoxic Hungate-type tubes (for strains demanding meat particles put those first into the tube (use 1 part meat particles to 4 or 5 parts fluid)). Autoclave at 121°C for 30 min. For agar slants use 15.0 g agar per 1000.0 ml medium.
  3. In some cases (as indicated in the catalogue) the addition of Haemin and Vitamin K1 or Vitamin K3 is necessary. Add to 1000 ml of medium after autoclaving: #8 and either #9 or #10.
Solution occurs in 7 other media (first defined in medium 78 )
Equipment needed: Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2 50 mg 0.5 -
3
(1 N)
1 ml 21.3 532.539
4 100 - -
Instructions:
  1. Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated.
Solution occurs in 5 other media (first defined in medium 78 )
Equipment needed: Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2 0.1 ml - -
3
(95 %)
20.0 ml 750.5 16291
Instructions:
  1. Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle.

SL 66: Vitamin K3 solution #

100
Solution occurs in 4 other media (first defined in medium 78 )
Equipment needed: Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5 g/l 5 -
2
(95 %)
x - -
3 100 ml 998.207 55408.8
Instructions:
  1. Dissolve 5 mg/ml of vitamin K3 in 95% ethanol, dilute to 0.05 mg/ml in water and filter sterilize. Store refrigerated in a brown bottle.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 230
Strains with modifications: 217
78 CHOPPED MEAT MEDIUM
a CHOPPED MEAT MEDIUM FOR TREPONEMA SP.
b MEDIUM FOR TREPONEMA PARVUM
c CHOPPED MEAT MEDIUM FOR FERTIBACTERIUM SP.

Gas composition

  • 100 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Ground beef 500 -
NaOH 53.25 1331.35
Casitone 30 -
Agar
optional
15 -
K2HPO4 5 28.707
Yeast extract 5 -
NaHCO3
optional
1 11.904
L-Cystein HCl 0.5 3.172
Resazurin 1.0e-3 0.004

Cultivation metadata from 230 strains BacDive

halophily slightly 4 strains
halophily not 4 strains
pH 6 - 8 19 strains
pH 8 - 10 9 strains
pH 4 - 6 7 strains
pH 10 - 14 1 strain
phyla 7 217 strains
oxygen anaerobe 176 strains
oxygen obligate anaerobe 12 strains
oxygen microaerophile 3 strains
oxygen aerotolerant 1 strain
temperature mesophilic 219 strains
temperature thermophilic 3 strains
temperature psychrophilic 1 strain

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