This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
MgSO4 x 7 H2O | 7.00 | g | 7 | 28.4 | |
MgCl2 x 6 H2O | 5.50 | g | 5.5 | 27.053 | |
KCl | 0.65 | g | 0.65 | 8.719 | |
NaBr | 0.10 | g | 0.1 | 0.972 | |
NaHCO3 | 2.00 | g | 2 | 23.808 | |
NH4Cl | 0.15 | g | 0.15 | 2.804 | |
K2HPO4 | 0.15 | g | 0.15 | 0.861 | |
CaCl2 x 2 H2O | 0.50 | g | 0.5 | 3.401 | |
Modified Wolin's mineral solution II | 10.00 | ml | - | - | |
Sulfur
(powdered) |
0.50 | g | 0.5 | 15.591 | |
Distilled water | 1000.00 | ml | - | - | |
1 Prepare the medium aerobically and adjust pH to 7.0 using H2SO4. Fill 20 ml medium into 100 ml serum bottles under H2/CO2 (80/20) gas and seal with a rubber stopper. Steam medium for 3 hours on each of 3 successive days. Before use add 100 µl of a sterile CaCO3 solution (10%). Give an atmosphere of H2/CO2 with an overpressure of two atmospheres and 20 ml filter-sterilized air. Incubate under shaking at 60°C. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid | 1.500 | g | 1.5 | 7.848 | |
MgSO4 x 7 H2O | 3.000 | g | 3 | 12.172 | |
MnSO4 x H2O | 0.500 | g | 0.5 | 2.958 | |
NaCl | 1.000 | g | 1 | 17.112 | |
FeSO4 x 7 H2O | 0.100 | g | 0.1 | 0.36 | |
CoSO4 x 7 H2O | 0.180 | g | 0.18 | 1.161 | |
CaCl2 x 2 H2O | 0.100 | g | 0.1 | 0.68 | |
ZnSO4 x 7 H2O | 0.180 | g | 0.18 | 0.626 | |
CuSO4 x 5 H2O | 0.010 | g | 0.01 | 0.04 | |
AlK(SO4)2 x 12 H2O | 0.020 | g | 0.02 | 0.042 | |
H3BO3 | 0.010 | g | 0.01 | 0.162 | |
Na2MoO4 x 2 H2O | 0.010 | g | 0.01 | 0.041 | |
NiCl2 x 6 H2O | 0.025 | g | 0.025 | 0.193 | |
Na2SeO3 x 5 H2O | 0.300 | mg | 3.0e-4 | 0.001 | |
Distilled water | 1000.000 | ml | - | - | |
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH. |
Last modified: | 22.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | 7.0 |
Equipment needed: | Filter |
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Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
MgCl2 x 6 H2O | 5.5 | 27.053 |
NaHCO3 | 2 | 23.808 |
KCl | 0.65 | 8.719 |
Sulfur | 0.5 | 15.591 |
NH4Cl | 0.15 | 2.804 |
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