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DSMZ

DSMZ 784 . NATRONIELLA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Natranaerobaculum magadiense DSM 24923

* Modification: Omit ethanol and add to the autoclaved medium 2.5 g/l Na2S2O3 x 5 H2O from an anoxic stock solution prepared under 100% N2 gas atmosphere and sterilized by filtration. Increase amount of yeast extract to 2.00 g/l.

Growth conditions: anaerobic 45 °C

Main sol. 784 #

ml
Equipment needed: Gassing station Autoclave Hungate tubes Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.2 g 0.199 1.459
MgCl2 x 6 H2O 0.1 g 0.099 0.488
NH4Cl 1.0 g 0.993 18.565
KCl 0.2 g 0.199 2.664
NaCl 16.0 g 15.889 271.882
Trace element solution SL-11 1.0 ml - -
Yeast extract* 2.0 g - -
Sodium resazurin
(0.1% w/v) 		0.5 ml 5.0e-4 0.002
Na2CO3 68.0 g 67.527 637.122
NaHCO3 38.0 g 37.736 449.199
Ethanol absolute* 5.0 ml - -
Wolin's vitamin solution (10x) 1.0 ml - -
Na2S x 9 H2O 1.0 g 0.993 4.135
Na2S2O3 x 5 H2O* 2.5 g - -
Distilled water 1000.0 ml - -
1 Dissolve ingredients except carbonate, bicarbonate, ethanol, vitamins and sulfide. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add and dissolve carbonates and sulfide while gassing the head space only. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before use add ethanol and vitamins from sterile anoxic stock solution prepared under 100% N2 gas. Vitamins are sterilized by filtration. Adjust pH of the complete medium to 9.5 - 10.0, if necessary.

Trace element solution SL-11 #

1000
Solution occurs in 22 other media (first defined in Medium 722).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA x 2 H2O 5.2 g 5.2 15.467
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 1000.0 ml - -
1 Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.

Wolin's vitamin solution (10x) #

1000
Solution occurs in 306 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 09.09.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.5 - 10.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 2
Strains with modifications: 1
Natranaerobaculum magadiense DSM 24923

  Yeast extract
  Ethanol absolute
  2.00 g Yeast extract
  2.50 g Na2S2O3 x 5 H2O

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
Na2CO3 67.527 637.122
NaHCO3 37.736 449.199
NaCl 15.889 271.882
Na2S2O3 x 5 H2O 2.483 10.003
Yeast extract 1.986 -
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Cultivation metadata from 2 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
pH 10 - 12 1 strain
phyla 1 2 strains
oxygen anaerobe 2 strains
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