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DSMZ

DSMZ 78 . CHOPPED MEAT MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Porphyromonas levii DSM 23370

* Modification: Plus haemine (5µg/ml) plus Vit K3; anaerobic

Growth conditions: 1-2 days anaerobic 37 °C pH 7.00

Main sol. 78 #

ml
Solution occurs in 4 other media (first defined in Medium 78).
Equipment needed: Hungate tubes Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Ground beef
(fat free) 		500.0 g 500 -
Distilled water 1000.0 ml - -
NaOH
(1 N) 		25.0 ml - -
1 Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water and NaOH, then boil for 15 min with stirring. Cool to room temperature, skim fat off surface, and filter, retaining both meat particles and filtrate. To the filtrate add water to a final volume of 1000 ml, and then add:
Casitone 30.0 g 30 -
Yeast extract 5.0 g 5 -
K2HPO4 5.0 g 5 28.707
Resazurin 1.0 mg 1.0e-3 0.004
2 To make medium anoxic bring it to a boil, cool under 100% N2 gas atmosphere, add 0.5 g/l L-cysteine hydrochloride and adjust pH to 7.0. Dispense under 100% N2 gas atmosphere by filling 7 ml medium into anoxic Hungate-type tubes (for strains demanding meat particles put those first into the tube (use 1 part meat particles to 4 or 5 parts fluid)). Autoclave at 121°C for 30 min. For agar slants use 15.0 g agar per 1000.0 ml medium.
L-Cysteine HCl 0.5 g 0.5 3.172
Agar , for solid medium 15.0 g 15 44.599
3 In some cases (as indicated in the catalogue) the addition of Haemin and Vitamin K1 or Vitamin K3 is necessary. Add to 1000 ml of medium after autoclaving:
Haemin solution
optional 		10.0 ml - -
Vitamin K1 solution , alternative
optional 		10.0 ml - -
Vitamin K3 solution , alternative
optional 		10.0 ml - -
Haemin* 5.0 mg/l - -

Haemin solution #

100
Solution occurs in 15 other media (first defined in Medium 78).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Haemin 50 mg 0.5 0.767
NaOH
(1 N) 		1 ml - -
Distilled water 100 ml - -
1 Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated.

Vitamin K1 solution #

20
Solution occurs in 12 other media (first defined in Medium 78).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin K1 0.1 ml - -
Ethanol
(95 %) 		20.0 ml 950 20620.8
1 Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle.

Vitamin K3 solution #

100
Solution occurs in 7 other media (first defined in Medium ).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin K3 5 mg 0.05 -
Ethanol
(95%) 		1 ml 9.5 206.208
Water 100 ml - -
1 Dissolve 5 mg vitamin K3 in 1 ml 95% ethanol, dilute to 0.05 mg/ml in water and filter sterilize. Store refrigerated in a brown bottle.

Metadata

Last modified: 21.09.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 267
Strains with modifications: 49
Porphyromonas levii DSM 23370

  5.0 mg/l Haemin

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
Ground beef 500 -
Casitone 30 -
Agar 15 44.599
Ethanol
optional 		9.5 206.208
K2HPO4 5 28.707
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Cultivation metadata from 267 strains BacDive

halophily non-halophilic 1 strain
pH 6 - 8 21 strains
pH 8 - 10 8 strains
pH 4 - 6 5 strains
phyla 9 263 strains
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