DSMZ 792c . NAUTILIA NITRATIREDUCENS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Gassing station Serum bottle Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 958 ml - -
Solution B 10 ml - -
Solution C 20 ml - -
Solution D 10 ml - -
Solution E 15 ml - -
1 Sparge solution A with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic serum vials (e.g., 20 ml medium in 100 ml bottles) and autoclave. Solutions B and C are autoclaved separately under 80% N2 and 20% CO2 gas mixture. Solution D is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solution E is autoclaved under 100% N2 gas atmosphere. To complete the medium appropriate amounts of solutions B to E are added to solution A. Adjust pH of the complete medium to 6.5, if necessary. After inoculation pressurize vials to 1 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.
2 Note: Solution A has to be prepared freshly. It cannot be stored for a long period of time.
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 20.00 g 20.877 357.235
MgSO4 x 7 H2O 3.50 g 3.653 14.823
MgCl2 x 6 H2O 2.75 g 2.871 14.12
CaCl2 x 2 H2O 0.75 g 0.783 5.325
KCl 0.33 g 0.344 4.621
NaBr 0.05 g 0.052 0.507
H3BO3 15.00 mg 0.016 0.253
SrCl2 x 6 H2O
(0.1% w/v)
7.00 ml 0.007 0.027
KI
(0.01% w/v)
0.50 ml 5.2e-5 2.0e-4
Wolfe's mineral elixir 1.00 ml - -
Distilled water 950.00 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
KNO3 1.0 g 100 989.09
KH2PO4 0.5 g 50 367.417
Distilled water 10.0 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 1 g 50 471.752
Distilled water 20 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
Wolin's vitamin solution 10 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S x 9 H2O 0.45 g 30 124.905
Distilled water 15.00 ml - -
Solution occurs in 23 other media (first defined in Medium 792).
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 30.00 g 30 121.716
MnSO4 x H2O 5.00 g 5 29.583
NaCl 10.00 g 10 171.116
FeSO4 x 7 H2O 1.00 g 1 3.597
CoCl2 x 6 H2O 1.80 g 1.8 13.863
CaCl2 x 2 H2O 1.00 g 1 6.802
ZnSO4 x 7 H2O 1.80 g 1.8 6.26
CuSO4 x 5 H2O 0.10 g 0.1 0.401
AlK(SO4)2 x 12 H2O 0.18 g 0.18 0.379
H3BO3 0.10 g 0.1 1.617
Na2MoO4 x 2 H2O 0.10 g 0.1 0.413
(NH4)2Ni(SO4)2 x 6 H2O 2.80 g 2.8 7.089
Na2WO4 x 2 H2O 0.10 g 0.1 0.303
Na2SeO4 0.10 g 0.1 0.529
Distilled water 1000.00 ml - -
1 First adjust pH to 1.0 with diluted H2SO4, then add and dissolve the salts.
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 30.06.22
Source: DSMZ
Medium type: Defined medium
Final pH: 6.5
Equipment needed: Autoclave, Filter, Gassing station, Serum bottle
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Gas composition

  • 80 % H2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 2.715 13.353
KNO3 0.987 9.764
Na2CO3 0.987 9.314
KH2PO4 0.494 3.627
Na2S x 9 H2O 0.444 1.85
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Cultivation metadata from 0 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
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