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793. MOORELLA GLYCERINI MEDIUM

Culture medium recipe

Vol.
×

M 793 #

ml
Equipment needed: Filter Autoclave Hungate tubes
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.33 g 0.325 2.387
2 0.33 g 0.325 6.072
3 0.33 g 0.325 4.357
4 0.33 g 0.325 1.598
5 0.33 g 0.325 2.209
6 0.50 g 0.492 -
7
(87%)
3.00 ml 3.237 35.147
8 Trace element solution SL-10 1.00 ml - -
9 Selenite-tungstate solution 1.00 ml - -
10
(0.1% w/v)
0.50 ml 4.9e-4 0.002
11 10.00 g 9.843 117.164
12 Vitamin solution 10.00 ml - -
13 0.50 g 0.492 2.049
14 1000.00 ml 982.487 54536.3
Instructions:
  1. Dissolve ingredients except bicarbonate, vitamins and sulfide, then sparge medium with 100% CO2 gas for 30 - 45 min to make it anoxic. Dissolve bicarbonate and adjust pH to 6.7, then dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins should be sterilized by filtration.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8

SL 1: Vitamin solution #

1000
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.7
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 100 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 994.267 55190.2
NaHCO3 9.843 117.164
Glycerol 3.237 35.147
Na2S x 9 H2O 0.492 2.049
Yeast extract 0.492 -
NH4Cl 0.325 6.072
KCl 0.325 4.357
KH2PO4 0.325 2.387
CaCl2 x 2 H2O 0.325 2.209
MgCl2 x 6 H2O 0.325 1.598
HCl 0.003 0.086
FeCl2 x 4 H2O 0.001 0.007
NaOH 4.9e-4 0.012
Na-resazurin 4.9e-4 0.002
CoCl2 x 6 H2O 1.9e-4 7.9e-4
MnCl2 x 4 H2O 9.8e-5 5.0e-4
Pyridoxine hydrochloride 9.8e-5 4.8e-4
ZnCl2 6.9e-5 5.1e-4
Nicotinic acid 4.9e-5 4.0e-4
p-aminobenzoic acid 4.9e-5 3.6e-4
Lipoic acid 4.9e-5 2.4e-4
Thiamine-HCl 4.9e-5 1.5e-4
Riboflavin 4.9e-5 1.3e-4
D-Ca-pantothenate 4.9e-5 1.0e-4
Na2MoO4 x 2 H2O 3.5e-5 1.5e-4
NiCl2 x 6 H2O 2.4e-5 9.9e-5
Biotin 2.0e-5 8.1e-5
Folic acid 2.0e-5 4.5e-5
H3BO3 5.9e-6 9.6e-5
Na-tungstate x 2 H2O 3.9e-6 1.2e-5
Na2SeO3 x 5 H2O 3.0e-6 1.1e-5
CuCl2 x 2 H2O 2.0e-6 1.2e-5
Vitamin B12 9.8e-7 6.2e-7

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain

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