DSMZ 794 . PSEUDOTHERMOTOGA HYPOGEA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.0 g 0.99 18.51
K2HPO4 0.3 g 0.297 1.705
KH2PO4 0.3 g 0.297 2.183
MgCl2 x 6 H2O 0.2 g 0.198 0.974
CaCl2 x 2 H2O 0.1 g 0.099 0.673
KCl 0.1 g 0.099 1.328
Na-acetate 0.5 g 0.495 6.035
NaCl 10.0 g 9.901 169.421
Yeast extract
(BD Bacto)
2.0 g 1.98 -
Trypticase peptone
(BD BBL)
2.0 g 1.98 -
Modified Wolin's mineral solution 10.0 ml - -
Sodium resazurin
(0.1% w/v)
0.5 ml 5.0e-4 0.002
L-Cysteine HCl x H2O 0.5 g 0.495 2.819
NaHCO3 2.0 g 1.98 23.572
D-Xylose 3.0 g 2.97 19.785
Na2S2O3 x 5 H2O 5.0 g 4.951 19.947
Na2S x 9 H2O 0.5 g 0.495 2.061
Distilled water 1000.0 ml - -
1 Dissolve ingredients (except cysteine, bicarbonate, xylose, thiosulfate and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add and dissolve cysteine, adjust pH to 6.5, then dispense medium under 80% N2 and 20% CO2 gas mixture into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add thiosulfate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Xylose is prepared under 100% N2 gas and sterilized by filtration. Adjust pH of complete medium to 7.0 - 7.2, if necessary.
Solution occurs in 166 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Last modified: 07.11.22
Source: DSMZ
Synonyms: THERMOTOGA HYPOGEA MEDIUM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0 - 7.2
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2S2O3 x 5 H2O 4.951 19.947
D-Xylose 2.97 19.785
NaHCO3 1.98 23.572
Yeast extract 1.98 -
Trypticase peptone 1.98 -
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain