795
795 .
STETTERIA MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 795 #
ml
Equipment needed: Gassing station
Serum bottle
Water bath
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Synthetic seawater (2 x conc.) | 500.00 | ml | - | - | |
| NaHCO3 | 0.16 | g | 0.157 | 1.871 | |
| KH2PO4 | 0.50 | g | 0.491 | 3.609 | |
| Modified Wolin's mineral solution | 15.00 | ml | - | - | |
|
NiCl2 x 6 H2O
(0.1% w/v) |
3.00 | ml | 0.003 | 0.023 | |
|
Peptone
(BD Bacto) |
2.00 | g | 1.965 | - | |
|
Yeast extract
(OXOID) |
1.00 | g | 0.982 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 4.9e-4 | 0.002 | |
|
Sulfur
, powdered
(powdered) |
10.00 | g | 9.823 | 306.304 | |
| Na2S x 9 H2O | 0.50 | g | 0.491 | 2.045 | |
| Distilled water | 500.00 | ml | - | - | |
| 1 Dissolve ingredients except sulfur and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into serum vials (e.g., 20 ml medium per 100 ml bottle) that contain already the appropriate amount of sulfur. After closing pressurize bottles to 2 bar overpressure using 80% H2 and 20% CO2 gas mixture, than heat at 100°C for 1.5 hours on each of 3 successive days. Before use add sulfide from a sterile anoxic stock solution prepared under 100% N2 gas atmosphere. Adjust pH of complete medium to 6.0, if necessary. Incubate without shaking. | |||||
Solution occurs in 167
other media (first defined in Medium 141).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
| MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
| MnSO4 x H2O | 0.50 | g | 0.5 | 2.958 | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
| CoSO4 x 7 H2O | 0.18 | g | 0.18 | 1.161 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| ZnSO4 x 7 H2O | 0.18 | g | 0.18 | 0.626 | |
| CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
| AlK(SO4)2 x 12 H2O | 0.02 | g | 0.02 | 0.042 | |
| H3BO3 | 0.01 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
| NiCl2 x 6 H2O | 0.03 | g | 0.03 | 0.231 | |
| Na2SeO3 x 5 H2O | 0.30 | mg | 3.0e-4 | 0.001 | |
| Na2WO4 x 2 H2O | 0.40 | mg | 4.0e-4 | 0.001 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH. | |||||
Solution occurs in 3
other media (first defined in Medium 795).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaCl | 55.40 | g | 55.4 | 947.981 |
| SrCl2 x 6 H2O | 0.03 | g | 0.03 | 0.113 |
| MgSO4 x 7 H2O | 14.00 | g | 14 | 56.801 |
| KI | 0.10 | mg | 1.0e-4 | 3.9e-4 |
| MgCl2 x 6 H2O | 11.00 | g | 11 | 54.106 |
| Na3-citrate | 20.00 | mg | 0.02 | 0.077 |
| KCl | 1.30 | g | 1.3 | 17.438 |
| CaCl2 x 2 H2O | 1.50 | g | 1.5 | 10.203 |
| NaBr | 0.20 | g | 0.2 | 1.944 |
| H3BO3 | 0.06 | g | 0.06 | 0.97 |
| Distilled water | 1000.00 | ml | - | - |
Metadata
| Last modified: | 06.04.22 |
| Source: | DSMZ |
| Taxonomic range: | Archaea |
| Medium type: | Complex medium |
| Final pH: | 6.0 |
| Equipment needed: | Gassing station, Serum bottle, Water bath |
| Edit in Medium builder | |
Gas composition
- 53 % H2
- 27 % N2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Sulfur | 9.823 | 306.304 |
| MgCl2 x 6 H2O | 5.403 | 26.575 |
| Peptone | 1.965 | - |
| Yeast extract | 0.982 | - |
| KCl | 0.639 | 8.565 |
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