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DSMZ

DSMZ 796 . ARCHAEOGLOBUS VENEFICUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 796 #

ml
Equipment needed: Autoclave Filter Gassing station Serum bottle
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 18.00 g 17.946 307.087
KCl 0.33 g 0.329 4.413
MgCl2 x 6 H2O 7.15 g 7.129 35.064
NH4Cl 0.25 g 0.249 4.66
CaCl2 x 2 H2O 0.14 g 0.14 0.949
K2HPO4 x 3 H2O 0.18 g 0.179 1.03
Trace element solution 1.00 ml - -
Fe(NH4)2(SO4)2 x 6 H2O
(0.1% w/v) 		2.00 ml 0.002 -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 0.002
NaHCO3 4.00 g 3.988 47.473
Na2SO3 1.00 g 0.997 7.91
Na-acetate 1.00 g 0.997 12.154
Na2S x 9 H2O 0.50 g 0.499 2.076
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except bicarbonate, sulfite, acetate and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Thereafter, add and dissolve bicarbonate and equilibrate the medium with the 80% N2 and 20% CO2 gas mixture to achieve a medium pH of 7.0. Dispense medium under same gas atmosphere into serum vials (e.g., 25 ml per 100 ml bottle) and autoclave. After sterilization add sulfite, acetate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. The stock solution of sulfite should be freshly prepared and sterilized by filtration. Prior to inoculation check pH of complete medium and adjust to 7.0, if necessary.
2 After inoculation, pressurize bottles to 1 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.

Trace element solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
MnCl2 x 4 H2O 5.8 g 5.8 29.307
NaCl 10.0 g 10 171.116
(NH4)2Ni(SO4)2 2.0 g 2 5.063
FeSO4 x 7 H2O 1.0 g 1 3.597
CoCl2 x 6 H2O 1.0 g 1 7.702
CaCl2 x 2 H2O 1.0 g 1 6.802
ZnSO4 x 7 H2O 1.0 g 1 3.478
CuSO4 x 5 H2O 0.1 g 0.1 0.401
H3BO3 0.1 g 0.1 1.617
AlK(SO4)2 x 12 H2O 0.1 g 0.1 0.211
Na2MoO4 x 2 H2O 0.1 g 0.1 0.413
Na2WO4 x 2 H2O 0.1 g 0.1 0.303
Na2SeO3 x 5 H2O 0.1 g 0.1 0.38
Distilled water 1000.0 ml - -
1 Adjust pH to 1.0 with HCl.

Metadata

Last modified: 27.10.22
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Defined medium
Final pH: 7.0
Equipment needed: Autoclave, Filter, Gassing station, Serum bottle
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Gas composition

  • 40 % N2
  • 40 % H2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 7.129 35.064
NaHCO3 3.988 47.473
Na-acetate 0.997 12.154
Na2SO3 0.997 7.91
Na2S x 9 H2O 0.499 2.076
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain