797
797 .
MODIFIED CHOPPED MEAT MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Dialister pneumosintes DSM 11619
This strain uses the standard medium without modifications.
Growth conditions: 2-3 days
anaerobic
37 °C
pH 7.00
Main sol. 797 #
ml
Equipment needed: Hungate tubes
Filter
Gassing station
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Ground beef
(fat free) |
500.0 | g | 500 | - | |
| Distilled water | 1000.0 | ml | - | - | |
|
NaOH
(1 N) |
25.0 | ml | - | - | |
| 1 Mix meat, water and NaOH, and bring to a boil with stirring. Cool to room temperature, skim fat off surface, and filter, retaining both meat particles and filtrate. To the filtrate add sufficient distilled water to restore volume to 1000 ml. To this filtrate add: | |||||
|
Trypticase
(BBL 11921) |
30.0 | g | 30 | - | |
| Yeast extract | 5.0 | g | 5 | - | |
| K2HPO4 | 5.0 | g | 5 | 28.707 | |
|
Resazurin
(0.025%) |
4.0 | ml | 1.0e-3 | 0.004 | |
| Agar , if required | 20.0 | g | 20 | 59.465 | |
| 2 Boil and cool medium under 80% N2, 10% H2, 10% CO2, and add: | |||||
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | 2.847 | |
| Haemin solution | 10.0 | ml | - | - | |
| Vitamin K1 solution | 0.2 | ml | - | - | |
| 3 Adjust medium for final pH 7.0. Anaerobically dispense 7 ml into tubes containing meat particles (use 1 part meat to 5 parts fluid) under the same gas phase. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Vitamin K1 | 0.15 | ml | - | - | |
|
Ethanol
(95%) |
30.00 | ml | 28.5 | 618.624 | |
| 1 Store solution in brown bottle under refrigeration. Discard after one month. | |||||
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Haemin | 50 | mg | 0.495 | 0.759 | |
|
NaOH
(1 N) |
1 | ml | - | - | |
| Distilled water | 100 | ml | - | - | |
| 1 Autoclave at 121°C for 15 min. | |||||
Metadata
| Last modified: | 19.04.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 80 % N2
- 10 % CO2
- 10 % H2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Dialister pneumosintes DSM 11619
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Ground beef | 500 | - |
| Trypticase | 30 | - |
| Agar | 20 | 59.465 |
| K2HPO4 | 5 | 28.707 |
| Yeast extract | 5 | - |
| Show more | ||
