Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Na2CO3 | 8.0 | g | ||
|
KCl | 0.2 | g | ||
|
NaCl | 10.0 | g | ||
|
NH4Cl | 0.5 | g | ||
|
K2HPO4 | 0.2 | g | ||
|
Yeast extract | 4.0 | g | ||
|
Trace element solution SL-10 | 1.0 | ml | ||
|
Wolin's vitamin solution | 1.0 | ml | ||
|
Na2S x 9 H2O | 0.5 | g | ||
|
Distilled water | 1000.0 | ml | ||
1 pH 9.0 | |||||
2 Prepare the medium anaerobically without the vitamins. Boil the medium, cool under N2 and dispense under N2 and autoclave the medium. Add the vitamin solution to the cooled, autoclaved medium. |
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
HCl
(25%) |
10.0 | ml | ||
|
FeCl2 x 4 H2O | 1.5 | g | ||
|
ZnCl2 | 70.0 | mg | ||
|
MnCl2 x 4 H2O | 100.0 | mg | ||
|
H3BO3 | 6.0 | mg | ||
|
CoCl2 x 6 H2O | 190.0 | mg | ||
|
CuCl2 x 2 H2O | 2.0 | mg | ||
|
NiCl2 x 6 H2O | 24.0 | mg | ||
|
Na2MoO4 x 2 H2O | 36.0 | mg | ||
|
Distilled water | 990.0 | ml | ||
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. |
Compound | Amount | Unit | |
---|---|---|---|
|
Biotin | 2.0 | mg |
|
Folic acid | 2.0 | mg |
|
Pyridoxine hydrochloride | 10.0 | mg |
|
Thiamine HCl | 5.0 | mg |
|
Riboflavin | 5.0 | mg |
|
Nicotinic acid | 5.0 | mg |
|
Calcium D-(+)-pantothenate | 5.0 | mg |
|
Vitamin B12 | 0.1 | mg |
|
p-Aminobenzoic acid | 5.0 | mg |
|
(DL)-alpha-Lipoic acid | 5.0 | mg |
|
Distilled water | 1000.0 | ml |