800
800 .
ANAEROBIOSPIRILLUM THOMASII MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 800 #
ml
Equipment needed: Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Wilkins Chalgrene Anaerobe Broth
(Oxoid CM 643) |
10.0 | ml | - | - | |
| 1 Use Wilkins Chalgrene Anaerobe Broth (Oxoid CM 643) and prepare it anaerobically under 80% N2 + 20% CO2 gas. After autoclaving and cooling to room temperature add per 10 ml the following sterile, anaerobic solutions: | |||||
|
5-Aminovaleric acid
(w/v) NaHCO3 (flushed with N2 + CO2) |
0.5 | ml | - | - | |
|
Fildes Enrichment
(BBL 20810) (under N2) |
1.0 | ml | - | - | |
|
3-Hydroxybenzoic acid
(w/v) cysteine-HCl x H2O (under N2) |
0.1 | ml | - | - | |
|
3-Hydroxybenzoic acid
(w/v) Na2S x 9 H2O (under N2) |
0.1 | ml | - | - | |
| 2 The pH of the completed medium should be 6.8 - 7.0. For growth an agar plates, Columbia sheep blood agar plates (BBL) may be used and incubated under an anaerobic atmosphere containig 5 - 10% CO2. | |||||
Metadata
| Last modified: | 31.03.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.9 |
| Equipment needed: | Autoclave, Gassing station |
| Edit in Medium builder | |
Gas composition
- 80 % N2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| 3-Hydroxybenzoic acid | - | - |
| Fildes Enrichment | - | - |
| 5-Aminovaleric acid | - | - |
| Wilkins Chalgrene Anaerobe Broth | - | - |
