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DSMZ

DSMZ 802 . GRACILIBACILLUS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 802 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 100.0 g/l 100 1711.16
MgSO4 x 7 H2O 20.0 g/l 20 81.144
KCl 2.0 g/l 2 26.828
NaBr 0.1 g/l 0.1 0.972
Yeast extract
(Difco) 		2.0 g/l 2 -
Trypticase peptone
(BBL) 		2.0 g/l 2 -
Tris-HCl 12.0 g/l 12 -
Trace metals solution 2.0 ml - -
Distilled water 998.0 ml - -
1 Adjust pH to 7.8 (with NaOH) before autoclaving. To the sterile medium, from sterile stock solutions, add 10 ml KH2PO4 (50 g/l), 5 ml CaCl2 (100 g/l), 2 ml FeCl2/MnCl2 (FeCl2 x 4 H2O, 20 g/l and MnCl2 x 4 H2O, 20 g/l), and glucose to a final concentration of 2 g/l.
KH2PO4
(50 g/l) 		10.0 ml 23.4 171.951
CaCl2
(100 g/l) 		5.0 ml - -
FeCl2/MnCl2 solution 2.0 ml - -
Glucose 2.0 g 2 11.101

Trace metals solution #

1010
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(32%) 		10 ml 3.2 87.767
FeCl2 x 4 H2O 2 g 1.98 9.96
CoCl2 x 6 H2O 250 mg 0.248 1.906
MnCl2 x 4 H2O 100 mg 0.099 0.5
ZnCl2 70 mg 0.069 0.508
H3BO3 6 mg 0.006 0.096
Na2MoO4 x 2 H2O 40 mg 0.04 0.164
NiCl2 x 6 H2O 70 mg 0.069 0.535
CuCl2 x 2 H2O 2 mg 0.002 0.012
AlCl3 x 6 H2O 60 mg 0.059 0.246
Na2WO4 x 2 H2O 6 mg 0.006 0.018
Distilled water 1000 ml - -
1 Dissolve salts in the HCl in the oder given above, then make up the solution to 1 litre with distilled water.

FeCl2/MnCl2 solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeCl2 x 4 H2O 20 g 20 100.598
MnCl2 x 4 H2O 20 g 20 101.059

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.8
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Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 100 1711.16
KH2PO4 23.4 171.951
MgSO4 x 7 H2O 20 81.144
Tris-HCl 12 -
KCl 2 26.828
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Cultivation metadata from 2 strains BacDive

halophily halotolerant 1 strain
pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 2 2 strains
nutrition chemoorganotroph 1 strain
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