804 .
HAEMOPHILUS MEDIUM
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Selected strain: Actinobacillus pleuropneumoniae DSM 13472
This strain uses the standard medium without modifications.
Growth conditions: 1-2 days
microaerobic
37 °C
pH 7.00
Equipment needed:
Filter
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Mueller-Hinton broth | 21 | g | ||
|
|
Yeast extract | 5 | g | ||
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|
Distilled water | 1000 | ml | ||
| 1 For Haemophilus spp., add 15 mg NAD and 15 mg hemine per L (X + V factors). | |||||
| 2 NAD stock solution: dissolve 1 mg NAD per ml dist. water, filter sterilize. | |||||
| 3 Hemine stock solution: dissolve 50 mg hemine in 1 ml 1 M NaOH, make up to 25 ml with dist. water, filter sterilize. | |||||