This strain uses the standard medium without modifications.
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Mueller-Hinton broth | 21 | g | ||
|
Yeast extract | 5 | g | ||
|
Distilled water | 1000 | ml | ||
1 For Haemophilus spp., add 15 mg NAD and 15 mg hemine per L (X + V factors). | |||||
2 NAD stock solution: dissolve 1 mg NAD per ml dist. water, filter sterilize. | |||||
3 Hemine stock solution: dissolve 50 mg hemine in 1 ml 1 M NaOH, make up to 25 ml with dist. water, filter sterilize. |