DSMZ 806 . THERMOCOCCUS MEDIUM

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Culture medium recipe

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Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
KCl 0.33 g 0.33 4.427
MgCl2 x 2 H2O 2.70 g 2.7 20.573
MgSO4 x 7 H2O 3.40 g 3.4 13.794
NH4Cl 0.25 g 0.25 4.674
CaCl2 x 2 H2O 0.14 g 0.14 0.952
K2HPO4 0.14 g 0.14 0.804
Na2SeO3 1.00 µg 1.0e-6 5.8e-6
NiCl2 x 6 H2O 1.00 µg 1.0e-6 7.7e-6
NaHCO3 1.00 g 1 11.904
NaCl 18.00 g 18 308.008
Resazurin 1.00 mg 1.0e-3 0.004
L-Cysteine HCl x H2O 0.50 g 0.5 2.847
Na2S x 9 H2O 0.50 g 0.5 2.082
Yeast extract 1.00 g 1 -
Trypticase 1.00 g 1 -
Sulphur 5.00 g 5 -
Modified Wolin's mineral solution II 10.00 ml - -
Wolin's vitamin solution 10.00 ml - -
Distilled water 980.00 ml - -
1 Prepare the medium without NaHCO3, cysteine hydrochloride, Na2S x 9 H2O, and vitamin solution. Boil the medium and cool under N2. Add the NaHCO3 to the cooled medium and adjust the pH to about pH 7.2. Sterilise the medium at 100°C for 3 hours on 3 consecutive days. Reduce the medium by adding 10% neutralised sodium sulphide and cysteine hydrochloride from sterile stock solutions. The vitamins are added from a sterile (filter sterilised) stock solution prepared under nitrogen. The final pH is 7.2.
Solution occurs in 22 other media (first defined in Medium 700).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.500 g - -
MgSO4 x 7 H2O 3.000 g - -
MnSO4 x H2O 0.500 g - -
NaCl 1.000 g - -
FeSO4 x 7 H2O 0.100 g - -
CoSO4 x 7 H2O 0.180 g - -
CaCl2 x 2 H2O 0.100 g - -
ZnSO4 x 7 H2O 0.180 g - -
CuSO4 x 5 H2O 0.010 g - -
AlK(SO4)2 x 12 H2O 0.020 g - -
H3BO3 0.010 g - -
Na2MoO4 x 2 H2O 0.010 g - -
NiCl2 x 6 H2O 0.025 g - -
Na2SeO3 x 5 H2O 0.300 mg - -
Distilled water 1000.000 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sulphur 5 -
MgCl2 x 2 H2O 2.7 20.573
NaHCO3 1 11.904
Trypticase 1 -
Yeast extract 1 -
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature hyperthermophilic 1 strain