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807. HALOANAEROBIUM ALCALIPHILUM MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 17.00 g 17 68.972
2 100.00 g 100 1711.08
3 Solution A 50.00 ml - -
4 Solution B 50.00 ml - -
5 Trace element solution SL-10 10.00 ml - -
6 1.00 mg 1.0e-3 0.004
7 10.00 g 10 -
8 10.00 g 10 -
9 5.00 g 5 27.754
10 0.25 g 0.25 1.041
11 4.10 g 4.1 48.806
12 0.50 g 0.5 2.847
13 890.00 ml 888.404 49313.9
Instructions:
  1. pH 7.0
  2. Prepare the medium without yeast extract, glucose, NaHCO3, cysteine hydrochloride and Na2S x 9 H2O. Boil and then cool under a gas atmosphere of N2:CO2 (80:20 v/v) and add the NaHCO3 and cysteine hydrochloride. Autoclave the medium and add from sterile stock solutions yeast extract, trypticase, glucose, and Na2S x 9 H2O. The final pH should be 7.0.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 6 g/l - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 6.00 g - -
2 6.00 g - -
3 12.00 g - -
4 2.60 g - -
5 2.50 g - -
6 0.28 g - -
7 0.28 g - -
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 898.286 49862.4
NaCl 100 1711.08
MgSO4 x 7 H2O 17 68.972
Yeast extract 10 -
Trypticase 10 -
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

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