DSMZ 807 . HALOANAEROBIUM ALCALIPHILUM MEDIUM

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Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 17.00 g 17 68.972
NaCl 100.00 g 100 1711.16
Solution A 50.00 ml - -
Solution B 50.00 ml - -
Trace element solution SL-10 10.00 ml - -
Resazurin 1.00 mg 1.0e-3 0.004
Yeast extract 10.00 g 10 -
Trypticase 10.00 g 10 -
Glucose 5.00 g 5 27.753
Na2S x 9 H2O 0.25 g 0.25 1.041
NaHCO3 4.10 g 4.1 48.806
L-Cysteine HCl x H2O 0.50 g 0.5 2.847
Distilled water 890.00 ml - -
1 pH 7.0
2 Prepare the medium without yeast extract, glucose, NaHCO3, cysteine hydrochloride and Na2S x 9 H2O. Boil and then cool under a gas atmosphere of N2:CO2 (80:20 v/v) and add the NaHCO3 and cysteine hydrochloride. Autoclave the medium and add from sterile stock solutions yeast extract, trypticase, glucose, and Na2S x 9 H2O. The final pH should be 7.0.
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 6 g/l - -
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 6.00 g - -
(NH4)2SO4 6.00 g - -
NaCl 12.00 g - -
MgSO4 x 7 H2O 2.60 g - -
NH4Cl 2.50 g - -
CaCl2 x 2 H2O 0.28 g - -
K2HPO4 0.28 g - -
Solution occurs in 263 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 100 1711.16
MgSO4 x 7 H2O 17 68.972
Trypticase 10 -
Yeast extract 10 -
Glucose 5 27.753
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain