808
808 .
SS1-MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 808 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaH2PO4 x 2 H2O | 3.93 | g | 3.93 | 32.756 | |
| K2HPO4 | 1.80 | g | 1.8 | 10.334 | |
| NH4Cl | 1.00 | g | 1 | 18.695 | |
| NaNO3 | 1.00 | g | 1 | 11.765 | |
| MgSO4 x 7 H2O | 0.20 | g | 0.2 | 0.811 | |
| CaCl2 | 0.01 | g | 0.01 | 0.09 | |
| Ferric EDTA solution | 1.00 | ml | - | - | |
| ZnSO4 x 7 H2O | 0.50 | g | 0.5 | 1.739 | |
| MnSO4 x H2O | 0.10 | mg | 1.0e-4 | 5.9e-4 | |
| CuSO4 x 5 H2O | 0.10 | mg | 1.0e-4 | 4.0e-4 | |
| Co(NO3)2 | 0.10 | mg | 1.0e-4 | 5.5e-4 | |
| Na2B4O7 x 10 H2O | 0.10 | mg | 1.0e-4 | - | |
| Na2MoO4 x 2 H2O | 2.00 | mg | 0.002 | 0.008 | |
| Distilled water | 990.00 | ml | - | - | |
|
Methanol
(filter-sterilized) |
10.00 | ml | 7.92 | 247.176 | |
| Agar , for solid medium | 15.00 | g | 15 | 44.599 | |
| 1 pH 6.8 | |||||
| 2 Autoclave the mineral salts solution and add 10 ml/l filter sterilised methanol to the cooled medium. Add 15 g/l agar for solid media. | |||||
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na-EDTA | 17.90 | g | 17.9 | 53.241 | |
| NaOH | 3.28 | g | 3.28 | 82.006 | |
| FeSO4 x 7 H2O | 13.70 | g | 13.7 | 49.278 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 1) Dissolve 17.9 g sodium EDTA and 3.28 g NaOH in 186 ml of distilled water. | |||||
| 2 2) Dissolve 13.7 g FeSO4 x 7 H2O in 364 ml of distilled ater, bubbling with air overnight, and store in a brown glass bottle. | |||||
| 3 3) Combine the two solutions and make up to 1 litre with distilled water. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Medium type: | Defined medium |
| Final pH: | 6.8 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |