812 .
SULFOBACILLUS DISULFIDOOXIDANS MEDIUM
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Equipment needed:
Autoclave
Filter
Final pH:
2.3
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
(NH4)2SO4 | 3.0 | g | ||
|
|
KCl | 0.1 | g | ||
|
|
KH2PO4 | 0.5 | g | ||
|
|
MgSO4 x 7 H2O | 0.5 | g | ||
|
|
Ca(NO3)2 x 4 H2O | 0.1 | g | ||
|
|
Yeast extract | 0.1 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
|
|
Glutathione
(10% w/v) |
10.0 | ml | ||
| 1 Adjust pH to 2.25 prior to autoclaving. Before use add per liter 10 ml of a filter sterilized 10% (w/v) glutathione solution. | |||||