Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2SO4 | 3.0 | g | 3 | 21.121 | |
CaCl2 x 2 H2O | 6.0 | g | 6 | 40.812 | |
NaCl | 40.0 | g | 40 | 684.463 | |
KCl | 2.0 | g | 2 | 26.828 | |
NH4Cl | 0.3 | g | 0.3 | 5.609 | |
KH2PO4 | 0.2 | g | 0.2 | 1.47 | |
MgCl2 x 6 H2O | 8.0 | g | 8 | 39.35 | |
SrCl2 x 6 H2O | 0.1 | g | 0.1 | 0.375 | |
Trace element solution SL-11 | 1.0 | ml | - | - | |
MOPS | 3.0 | g | 3 | 14.336 | |
Yeast extract | 1.0 | g | 1 | - | |
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | - | |
Iron powder
(or nails) |
100.0 | g | 100 | 1790.67 | |
Na-L-lactate | 3.6 | g | 3.6 | - | |
Na2CO3 | 0.5 | g | 0.5 | 4.718 | |
Distilled water | 1000.0 | ml | - | - | |
1 Dissolve ingredients except iron, lactate and carbonate, adjust pH to 6.0 with NaOH and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials containing 1 - 2 g iron powder or an iron nail. Autoclave for 30 min at 105°C. Before use add lactate from a sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Final medium pH should be 7.2. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2-EDTA x 2 H2O | 5.2 | g | 5.2 | 15.467 | |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
Distilled water | 1000.0 | ml | - | - | |
1 Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml. |
Last modified: | 07.04.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.2 |
Equipment needed: | Autoclave, Gassing station, Hungate tubes |
Edit in Medium builder |
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Iron powder | 100 | 1790.67 |
NaCl | 40 | 684.463 |
MgCl2 x 6 H2O | 8 | 39.35 |
CaCl2 x 2 H2O | 6 | 40.812 |
Na-L-lactate | 3.6 | - |
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