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815a. DESULFOCELLA HALOPHILA MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 3.0 g 2.994 21.078
2 6.0 g 5.988 40.731
3 40.0 g 39.92 683.067
4 2.0 g 1.996 26.774
5 0.3 g 0.299 5.597
6 0.2 g 0.2 1.467
7 8.0 g 7.984 39.272
8 0.1 g 0.1 0.374
9 Trace element solution SL-11 1.0 ml - -
10 3.0 g 2.994 -
11 1.0 g 0.998 -
12
(0.1% w/v)
0.5 ml 5.0e-4 0.002
13 2.5 g 2.495 22.673
14 0.5 g 0.499 4.708
15 0.1 g 0.1 0.416
16 1000.0 ml 996.215 55298.2
18 , may stimulate growth of some strains 20.0 mg/l 0.02 -
Instructions:
  1. Dissolve ingredients except pyruvate, bicarbonate and sulfide, adjust pH to 6.0 with NaOH and sparge medium for 30 - 45 min with 100% N2 gas to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before use add pyruvate (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Final medium pH should be 7.2.
  2. Note: Prior to inoculation 10- 20 mg/l sodium dithionite (added from a 5% w/v solution freshly prepared under N2 and filter-sterilized) can be added to the medium to stimulate growth at the beginning.
Solution occurs in 22 other media (first defined in medium 722 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.2 g 5.2 13.97
2 1.5 g 1.5 7.545
3 70.0 mg 0.07 0.514
4 100.0 mg 0.1 0.505
5 6.0 mg 0.006 0.097
6 190.0 mg 0.19 0.799
7 2.0 mg 0.002 0.012
8 24.0 mg 0.024 0.101
9 36.0 mg 0.036 0.149
10 1000.0 ml 998.207 55408.8
Instructions:
  1. Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 997.211 55353.5
NaCl 39.92 683.067
MgCl2 x 6 H2O 7.984 39.272
CaCl2 x 2 H2O 5.988 40.731
Na2SO4 2.994 21.078
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

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