DSMZ 817 . THERMOSPHAERA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 2.2 g 2.2 10.821
NaCl 0.9 g 0.9 15.4
KCl 17.0 mg 0.017 0.228
NH4Cl 12.0 mg 0.012 0.224
CaCl2 x 2 H2O
(0.1% w/v)
7.0 ml 0.007 0.048
K2HPO4 x 3 H2O
(0.1% w/v)
7.0 ml 0.007 0.04
FeCl3
(0.01% w/v in 0.2 N HCl)
0.5 ml 5.0e-5 3.1e-4
Yeast extract
(BD Bacto)
1.0 g 1 -
Peptone
(BD Bacto)
1.0 g 1 -
Sodium resazurin
(0.1% w/v)
0.5 ml 5.0e-4 -
Na2CO3 0.5 g 0.5 4.718
Wolin's vitamin solution 10.0 ml - -
Na2S x 9 H2O 0.5 g 0.5 2.082
Distilled water 1000.0 ml - -
1 Dissolve ingredients except carbonate, vitamins and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Vitamins should be sterilized by filtration. The pH of the complete medium should be adjusted to 6.5. After inoculation pressurize tubes or bottles (heavy walled) to 2 bar overpressure with sterile 80% N2 and 20% CO2 gas mixture.
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 07.04.22
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 6.5
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 2.2 10.821
Yeast extract 1 -
Peptone 1 -
NaCl 0.9 15.4
Na2CO3 0.5 4.718
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
nutrition heterotroph 1 strain
oxygen anaerobe 1 strain
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