817
817 .
THERMOSPHAERA MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Thermosphaera aggregans DSM 11486
This strain uses the standard medium without modifications.
Growth conditions: anaerobic
85 °C
Main sol. 817 #
ml
Equipment needed: Filter
Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MgCl2 x 6 H2O | 2.2 | g | 2.167 | 10.661 | |
| NaCl | 0.9 | g | 0.887 | 15.173 | |
| KCl | 17.0 | mg | 0.017 | 0.225 | |
| NH4Cl | 12.0 | mg | 0.012 | 0.221 | |
|
CaCl2 x 2 H2O
(0.1% w/v) |
7.0 | ml | 0.007 | 0.047 | |
|
K2HPO4 x 3 H2O
(0.1% w/v) |
7.0 | ml | 0.007 | 0.04 | |
|
FeCl3
(0.01% w/v in 0.2 N HCl) |
0.5 | ml | 4.9e-5 | 3.0e-4 | |
|
Yeast extract
(BD Bacto) |
1.0 | g | 0.985 | - | |
|
Peptone
(BD Bacto) |
1.0 | g | 0.985 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 4.9e-4 | 0.002 | |
| Na2CO3 | 0.5 | g | 0.493 | 4.648 | |
| Wolin's vitamin solution (10x) | 1.0 | ml | - | - | |
| Na2S x 9 H2O | 0.5 | g | 0.493 | 2.051 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients except carbonate, vitamins and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. Add vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Vitamins should be sterilized by filtration. The pH of the complete medium should be adjusted to 6.5. | |||||
| 2 After inoculation pressurize tubes or bottles (heavy walled) to 2 bar overpressure with sterile 80% N2 and 20% CO2 gas mixture. | |||||
Solution occurs in 305
other media (first defined in Medium 120).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 20 | mg | 0.02 | 0.082 |
| Folic acid | 20 | mg | 0.02 | 0.045 |
| Pyridoxine hydrochloride | 100 | mg | 0.1 | 0.486 |
| Thiamine HCl | 50 | mg | 0.05 | 0.148 |
| Riboflavin | 50 | mg | 0.05 | 0.133 |
| Nicotinic acid | 50 | mg | 0.05 | 0.406 |
| Calcium D-(+)-pantothenate | 50 | mg | 0.05 | 0.21 |
| Vitamin B12 | 1 | mg | 1.0e-3 | 7.4e-4 |
| p-Aminobenzoic acid | 50 | mg | 0.05 | 0.365 |
| (DL)-alpha-Lipoic acid | 50 | mg | 0.05 | 0.242 |
| Distilled water | 1000 | ml | - | - |
Metadata
| Last modified: | 27.10.22 |
| Source: | DSMZ |
| Taxonomic range: | Archaea |
| Medium type: | Complex medium |
| Final pH: | 6.5 |
| Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 80 % N2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Thermosphaera aggregans DSM 11486
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| MgCl2 x 6 H2O | 2.167 | 10.661 |
| Yeast extract | 0.985 | - |
| Peptone | 0.985 | - |
| NaCl | 0.887 | 15.173 |
| Na2CO3 | 0.493 | 4.648 |
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