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81a. THIOMONAS ISLANDICA MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Anaerobic workbench Autoclave Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.30 g 2.275 13.061
2 2.90 g 2.868 16.116
3 1.00 g 0.989 18.491
4 0.01 g 0.01 0.067
5 0.50 g 0.495 2.007
6
(0.1% w/v)
5.00 ml 0.005 0.035
7 Trace element solution SL-6 5.00 ml - -
8 0.50 g 0.495 5.887
9 0.05 g 0.049 -
10 Seven vitamins solution 1.00 ml - -
11 1000.00 ml 987.346 54806
Instructions:
  1. Dissolve ingredients (ecxept bicarbonate, ferric ammonium citrate and vitamins), then sparge solution with 80% N2 and 20% CO2 gas mixture for at least 30 - 45 min to remove dissolved oxygen and to saturate the solution with CO2. Adjust pH to 4.5 and dispense solution under air atmosphere into serum vials suitable for anaerobic cultivation to 50% of volume. Close vials with butyl rubber septa to prevent free exchange of oxygen with the external atmosphere and autoclave. Add ferric ammonium citrate and vitamins from anoxic stock solutions sterilized by filtration and bicarbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Adjust pH of the complete medium to 5.0, if necessary. Prior to inoculation add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure.
  2. Note: Use at least 10%(v/v) as inoculum.
Solution occurs in 38 other media (first defined in medium 1142 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.10 g 0.1 0.348
2 0.03 g 0.03 0.152
3 0.30 g 0.3 4.852
4 0.20 g 0.2 0.841
5 0.01 g 0.01 0.059
6 0.02 g 0.02 0.084
7 0.03 g 0.03 0.124
8 1000.00 ml 998.207 55408.8
Solution occurs in 86 other media (first defined in medium 503 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 100 mg 0.1 0.063
2 80 mg 0.08 0.583
3 20 mg 0.02 0.082
4 200 mg 0.2 1.625
5 100 mg 0.1 0.21
6 300 mg 0.3 1.459
7 200 mg 0.2 0.536
8 1000 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 5.0
Equipment needed: Anaerobic workbench, Autoclave, Filter

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 20 % CO2
  • 80 % H2

Light

1 strain needs light for growth. Select a strain for more details.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 993.27 55134.8
Na2HPO4 x 2 H2O 2.868 16.116
K2HPO4 2.275 13.061
NH4Cl 0.989 18.491
NaHCO3 0.495 5.887
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Cultivation metadata from 1 strains BacDive

pH 2 - 4 1 strain
pH 4 - 6 1 strain
phyla 1 1 strain
oxygen aerobe 1 strain
oxygen microaerophile 1 strain
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