Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NH4Cl | 1.0 | g | 0.989 | 18.492 | |
K2HPO4 | 0.3 | g | 0.297 | 1.704 | |
KH2PO4 | 0.3 | g | 0.297 | 2.181 | |
MgCl2 x 6 H2O | 0.2 | g | 0.198 | 0.973 | |
CaCl2 x 2 H2O | 0.1 | g | 0.099 | 0.673 | |
KCl | 0.1 | g | 0.099 | 1.327 | |
NaCl | 0.6 | g | 0.593 | 10.155 | |
Modified Wolin's mineral solution | 10.0 | ml | - | - | |
Na-acetate | 0.5 | g | 0.495 | 6.029 | |
Yeast extract | 1.0 | g | 0.989 | - | |
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 4.9e-4 | 0.002 | |
NaHCO3 | 4.0 | g | 3.956 | 47.097 | |
Wolin's vitamin solution (10x) | 1.0 | ml | - | - | |
L-Cysteine HCl x H2O | 0.5 | g | 0.495 | 2.816 | |
Na2S x 9 H2O | 0.5 | g | 0.495 | 2.059 | |
Distilled water | 1000.0 | ml | - | - | |
1 Dissolve ingredients except bicarbonate, vitamins, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add and dissolve bicarbonate, adjust pH to 7.0, then dispense medium under 80% H2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. Add vitamins (sterilized by filtration), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to use check pH of complete medium and adjust to 6.8 - 7.0, if necessary. | |||||
2 After inoculation pressurize cultivation vessels to 2 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
MnSO4 x H2O | 0.50 | g | 0.5 | 2.958 | |
NaCl | 1.00 | g | 1 | 17.112 | |
FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
CoSO4 x 7 H2O | 0.18 | g | 0.18 | 1.161 | |
CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
ZnSO4 x 7 H2O | 0.18 | g | 0.18 | 0.626 | |
CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
AlK(SO4)2 x 12 H2O | 0.02 | g | 0.02 | 0.042 | |
H3BO3 | 0.01 | g | 0.01 | 0.162 | |
Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
NiCl2 x 6 H2O | 0.03 | g | 0.03 | 0.231 | |
Na2SeO3 x 5 H2O | 0.30 | mg | 3.0e-4 | 0.001 | |
Na2WO4 x 2 H2O | 0.40 | mg | 4.0e-4 | 0.001 | |
Distilled water | 1000.00 | ml | - | - | |
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 20 | mg | 0.02 | 0.082 |
Folic acid | 20 | mg | 0.02 | 0.045 |
Pyridoxine hydrochloride | 100 | mg | 0.1 | 0.486 |
Thiamine HCl | 50 | mg | 0.05 | 0.148 |
Riboflavin | 50 | mg | 0.05 | 0.133 |
Nicotinic acid | 50 | mg | 0.05 | 0.406 |
Calcium D-(+)-pantothenate | 50 | mg | 0.05 | 0.21 |
Vitamin B12 | 1 | mg | 1.0e-3 | 7.4e-4 |
p-Aminobenzoic acid | 50 | mg | 0.05 | 0.365 |
(DL)-alpha-Lipoic acid | 50 | mg | 0.05 | 0.242 |
Distilled water | 1000 | ml | - | - |
Last modified: | 27.10.22 |
Source: | DSMZ |
Taxonomic range: | Archaea |
Medium type: | Complex medium |
Final pH: | 6.8 - 7.0 |
Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
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Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaHCO3 | 3.956 | 47.097 |
NH4Cl | 0.989 | 18.492 |
Yeast extract | 0.989 | - |
Na-acetate | 0.495 | 6.029 |
L-Cysteine HCl x H2O | 0.495 | 2.816 |
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pH | 4 - 6 | 1 strain |
pH | 6 - 8 | 1 strain |
pH | 8 - 10 | 1 strain |
phyla | 1 | 18 strains |
oxygen | anaerobe | 18 strains |
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